Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8089 | 5' | -54.3 | NC_001978.2 | + | 41440 | 0.66 | 0.64324 |
Target: 5'- -cGGACGcuugccGAAG-UCCAUUGCCGUCUUc -3' miRNA: 3'- acUCUGU------CUUCgGGGUAACGGCAGAA- -5' |
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8089 | 5' | -54.3 | NC_001978.2 | + | 33704 | 0.66 | 0.636318 |
Target: 5'- cGAGACGGAgacgccgaagugaucAGUgCCGUaaucaucgaucUGCCGUCg- -3' miRNA: 3'- aCUCUGUCU---------------UCGgGGUA-----------ACGGCAGaa -5' |
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8089 | 5' | -54.3 | NC_001978.2 | + | 729 | 0.66 | 0.608643 |
Target: 5'- cUGAGGCuGAGGCCaCCcgcGCCGUgUa -3' miRNA: 3'- -ACUCUGuCUUCGG-GGuaaCGGCAgAa -5' |
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8089 | 5' | -54.3 | NC_001978.2 | + | 4384 | 0.67 | 0.562869 |
Target: 5'- cUGGGGCAGggGCgCCGUcGCUG-Cg- -3' miRNA: 3'- -ACUCUGUCuuCGgGGUAaCGGCaGaa -5' |
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8089 | 5' | -54.3 | NC_001978.2 | + | 36737 | 0.69 | 0.4233 |
Target: 5'- gGAGACGGc-GCCCCAg-GCCGUUc- -3' miRNA: 3'- aCUCUGUCuuCGGGGUaaCGGCAGaa -5' |
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8089 | 5' | -54.3 | NC_001978.2 | + | 29643 | 0.71 | 0.366195 |
Target: 5'- --uGACAGAAGCCCCGUUaCCGa--- -3' miRNA: 3'- acuCUGUCUUCGGGGUAAcGGCagaa -5' |
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8089 | 5' | -54.3 | NC_001978.2 | + | 1839 | 0.74 | 0.24125 |
Target: 5'- aGcGGCAGGguucaAGCCCUGUUGCCGUCc- -3' miRNA: 3'- aCuCUGUCU-----UCGGGGUAACGGCAGaa -5' |
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8089 | 5' | -54.3 | NC_001978.2 | + | 9537 | 1.06 | 0.001105 |
Target: 5'- cUGAGACAGAAGCCCCAUUGCCGUCUUa -3' miRNA: 3'- -ACUCUGUCUUCGGGGUAACGGCAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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