Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
809 | 3' | -46.2 | NC_000866.4 | + | 82671 | 0.66 | 0.999945 |
Target: 5'- -uUGCuUCUGGc-GAugUUACAGAAa -3' miRNA: 3'- ucACGuAGACCuuCUugAAUGUCUUa -5' |
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809 | 3' | -46.2 | NC_000866.4 | + | 74437 | 0.67 | 0.999831 |
Target: 5'- -aUGCG-CUGGAAGAACUU-CAGc-- -3' miRNA: 3'- ucACGUaGACCUUCUUGAAuGUCuua -5' |
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809 | 3' | -46.2 | NC_000866.4 | + | 137175 | 0.81 | 0.692984 |
Target: 5'- uAGUGCAUCUGGAAGuAACcaauCAGAAUa -3' miRNA: 3'- -UCACGUAGACCUUC-UUGaau-GUCUUA- -5' |
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809 | 3' | -46.2 | NC_000866.4 | + | 137421 | 1.06 | 0.034798 |
Target: 5'- aAGUGCAUCUGGAAGAACUUACAGAAUu -3' miRNA: 3'- -UCACGUAGACCUUCUUGAAUGUCUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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