Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8091 | 5' | -56.7 | NC_001978.2 | + | 34344 | 0.66 | 0.564879 |
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Target: 5'- uUCGCGCgucgUGUacagcguGGCUCCGGcaagcuCCGcCGa -3' miRNA: 3'- cAGCGCGa---ACA-------UCGAGGCCu-----GGCaGU- -5' |
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| 8091 | 5' | -56.7 | NC_001978.2 | + | 33560 | 0.73 | 0.197344 |
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Target: 5'- uGUCGCcCUUGUcGGCgCCGuGACCGUCGg -3' miRNA: 3'- -CAGCGcGAACA-UCGaGGC-CUGGCAGU- -5' |
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| 8091 | 5' | -56.7 | NC_001978.2 | + | 26186 | 0.69 | 0.392542 |
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Target: 5'- -aCGCGCUUGUGGCa-UGGACUGaCGg -3' miRNA: 3'- caGCGCGAACAUCGagGCCUGGCaGU- -5' |
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| 8091 | 5' | -56.7 | NC_001978.2 | + | 20390 | 0.68 | 0.401793 |
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Target: 5'- cUCGCGCcgUGcgGGCUUCcuuGACCGUCGa -3' miRNA: 3'- cAGCGCGa-ACa-UCGAGGc--CUGGCAGU- -5' |
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| 8091 | 5' | -56.7 | NC_001978.2 | + | 20166 | 0.68 | 0.420704 |
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Target: 5'- -aCGgGCgUGUAGCUCUucugacuGACCGUCAu -3' miRNA: 3'- caGCgCGaACAUCGAGGc------CUGGCAGU- -5' |
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| 8091 | 5' | -56.7 | NC_001978.2 | + | 11633 | 1.08 | 0.000613 |
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Target: 5'- uGUCGCGCUUGUAGCUCCGGACCGUCAc -3' miRNA: 3'- -CAGCGCGAACAUCGAGGCCUGGCAGU- -5' |
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| 8091 | 5' | -56.7 | NC_001978.2 | + | 5098 | 0.7 | 0.323689 |
|
Target: 5'- -cCGCGCUUGa---UCCGG-CCGUCAa -3' miRNA: 3'- caGCGCGAACaucgAGGCCuGGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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