Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8096 | 5' | -51.1 | NC_001978.2 | + | 29611 | 0.66 | 0.832748 |
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Target: 5'- ----uGcCCGUACAUCUGAAGGaUGAc- -3' miRNA: 3'- cacuuC-GGCAUGUGGACUUCC-ACUaa -5' |
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| 8096 | 5' | -51.1 | NC_001978.2 | + | 31310 | 0.66 | 0.823052 |
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Target: 5'- uUGgcGCCG-ACGCgCUGAGuGGUGAc- -3' miRNA: 3'- cACuuCGGCaUGUG-GACUU-CCACUaa -5' |
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| 8096 | 5' | -51.1 | NC_001978.2 | + | 17645 | 0.67 | 0.760496 |
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Target: 5'- -cGGAGCCGU-CGCCcGGuacaAGGUGAc- -3' miRNA: 3'- caCUUCGGCAuGUGGaCU----UCCACUaa -5' |
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| 8096 | 5' | -51.1 | NC_001978.2 | + | 22693 | 0.67 | 0.749463 |
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Target: 5'- -aGAAGCUGUGCGCCguucuGAGGUaGUUg -3' miRNA: 3'- caCUUCGGCAUGUGGac---UUCCAcUAA- -5' |
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| 8096 | 5' | -51.1 | NC_001978.2 | + | 8623 | 0.67 | 0.749463 |
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Target: 5'- cUGAAGCUGa--ACCUGAAGGgGAa- -3' miRNA: 3'- cACUUCGGCaugUGGACUUCCaCUaa -5' |
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| 8096 | 5' | -51.1 | NC_001978.2 | + | 39563 | 0.68 | 0.680888 |
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Target: 5'- -cGcuGCCGU-CGCCgGAAGGUGGUc -3' miRNA: 3'- caCuuCGGCAuGUGGaCUUCCACUAa -5' |
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| 8096 | 5' | -51.1 | NC_001978.2 | + | 21120 | 0.74 | 0.354088 |
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Target: 5'- -aGAcGGCCG-ACGCgCUGAAGGUGAUUc -3' miRNA: 3'- caCU-UCGGCaUGUG-GACUUCCACUAA- -5' |
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| 8096 | 5' | -51.1 | NC_001978.2 | + | 40386 | 0.75 | 0.327706 |
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Target: 5'- --cGAGCCGUugACCUGggGGaagUGAUg -3' miRNA: 3'- cacUUCGGCAugUGGACuuCC---ACUAa -5' |
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| 8096 | 5' | -51.1 | NC_001978.2 | + | 14595 | 1.07 | 0.002216 |
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Target: 5'- cGUGAAGCCGUACACCUGAAGGUGAUUg -3' miRNA: 3'- -CACUUCGGCAUGUGGACUUCCACUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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