Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8097 | 5' | -57 | NC_001978.2 | + | 20481 | 0.66 | 0.499602 |
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Target: 5'- uUGaaGUCUUCGgcgacgucGGCcGACCGGCGCGa -3' miRNA: 3'- uACgcCAGAAGC--------CCGuCUGGUUGCGU- -5' |
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| 8097 | 5' | -57 | NC_001978.2 | + | 20424 | 0.66 | 0.489058 |
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Target: 5'- --uCGG-CUUcCGGGguGACCGGCGUc -3' miRNA: 3'- uacGCCaGAA-GCCCguCUGGUUGCGu -5' |
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| 8097 | 5' | -57 | NC_001978.2 | + | 2897 | 0.69 | 0.321072 |
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Target: 5'- cUGCacUCUUCGGGCGGGggcacuccCCAGCGCu -3' miRNA: 3'- uACGccAGAAGCCCGUCU--------GGUUGCGu -5' |
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| 8097 | 5' | -57 | NC_001978.2 | + | 35657 | 0.7 | 0.305293 |
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Target: 5'- -cGgGGUCUUCGGuCGcGCCGACGCu -3' miRNA: 3'- uaCgCCAGAAGCCcGUcUGGUUGCGu -5' |
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| 8097 | 5' | -57 | NC_001978.2 | + | 11219 | 0.7 | 0.290116 |
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Target: 5'- -gGCGGag-UCGGGCAGACCcAUGaCAa -3' miRNA: 3'- uaCGCCagaAGCCCGUCUGGuUGC-GU- -5' |
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| 8097 | 5' | -57 | NC_001978.2 | + | 20649 | 0.73 | 0.194739 |
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Target: 5'- -cGCcGUCaggUCGGGCAGACCGGuCGCc -3' miRNA: 3'- uaCGcCAGa--AGCCCGUCUGGUU-GCGu -5' |
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| 8097 | 5' | -57 | NC_001978.2 | + | 14802 | 1.07 | 0.000592 |
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Target: 5'- aAUGCGGUCUUCGGGCAGACCAACGCAg -3' miRNA: 3'- -UACGCCAGAAGCCCGUCUGGUUGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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