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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 81 | 5' | -57.1 | AC_000005.1 | + | 24731 | 0.66 | 0.421131 |
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Target: 5'- uUCGCAGCaUGUccccgaaaGCCGCAAaacaGGCUAUUg -3' miRNA: 3'- -GGCGUCG-ACGc-------CGGCGUUg---UCGAUAA- -5' |
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| 81 | 5' | -57.1 | AC_000005.1 | + | 11604 | 0.66 | 0.431065 |
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Target: 5'- cCCGCGGUuuugGCGGCCauGCAAagcCAGCc--- -3' miRNA: 3'- -GGCGUCGa---CGCCGG--CGUU---GUCGauaa -5' |
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| 81 | 5' | -57.1 | AC_000005.1 | + | 9701 | 0.66 | 0.451337 |
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Target: 5'- uCCGUAGCcuugcaGCGGuuGUAAUaaAGCUAa- -3' miRNA: 3'- -GGCGUCGa-----CGCCggCGUUG--UCGAUaa -5' |
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| 81 | 5' | -57.1 | AC_000005.1 | + | 23219 | 0.66 | 0.431065 |
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Target: 5'- gCGCGGCUGCGGUUaGCucGACAGg---- -3' miRNA: 3'- gGCGUCGACGCCGG-CG--UUGUCgauaa -5' |
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| 81 | 5' | -57.1 | AC_000005.1 | + | 10512 | 0.67 | 0.354762 |
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Target: 5'- aCCGCuGCUGaGGCCGUggUugcccugGGCUGa- -3' miRNA: 3'- -GGCGuCGACgCCGGCGuuG-------UCGAUaa -5' |
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| 81 | 5' | -57.1 | AC_000005.1 | + | 902 | 0.69 | 0.282501 |
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Target: 5'- uCCGCAGCUGCuGCUGCc---GCUGa- -3' miRNA: 3'- -GGCGUCGACGcCGGCGuuguCGAUaa -5' |
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| 81 | 5' | -57.1 | AC_000005.1 | + | 3636 | 0.75 | 0.102441 |
|
Target: 5'- gCCGUAGaaGCGGCCGCGGaAGCUGc- -3' miRNA: 3'- -GGCGUCgaCGCCGGCGUUgUCGAUaa -5' |
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| 81 | 5' | -57.1 | AC_000005.1 | + | 15384 | 0.75 | 0.102441 |
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Target: 5'- -aGCuGUUGCGGCCGCAGCuGCg--- -3' miRNA: 3'- ggCGuCGACGCCGGCGUUGuCGauaa -5' |
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| 81 | 5' | -57.1 | AC_000005.1 | + | 3575 | 0.78 | 0.065469 |
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Target: 5'- gCUGCAGCUuccGCGGCCGCuucuACGGCUc-- -3' miRNA: 3'- -GGCGUCGA---CGCCGGCGu---UGUCGAuaa -5' |
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| 81 | 5' | -57.1 | AC_000005.1 | + | 15328 | 1.07 | 0.000349 |
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Target: 5'- gCCGCAGCUGCGGCCGCAACAGCUAUUg -3' miRNA: 3'- -GGCGUCGACGCCGGCGUUGUCGAUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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