Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8104 | 3' | -54.6 | NC_001978.2 | + | 29174 | 0.66 | 0.648039 |
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Target: 5'- cCCcguGCCgCGCGCUUCAcGCUGCcaaCGCg -3' miRNA: 3'- -GGau-CGG-GUGCGAGGUuCGAUGa--GUG- -5' |
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| 8104 | 3' | -54.6 | NC_001978.2 | + | 37237 | 0.67 | 0.566504 |
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Target: 5'- aCCUAGgCaucgggcggacgGCGCUCCGucAGgUGCUCGCg -3' miRNA: 3'- -GGAUCgGg-----------UGCGAGGU--UCgAUGAGUG- -5' |
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| 8104 | 3' | -54.6 | NC_001978.2 | + | 73 | 0.69 | 0.45043 |
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Target: 5'- aCCUAGCgCGCGUuuUCCGAGCUGg---- -3' miRNA: 3'- -GGAUCGgGUGCG--AGGUUCGAUgagug -5' |
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| 8104 | 3' | -54.6 | NC_001978.2 | + | 222 | 0.71 | 0.382542 |
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Target: 5'- uCCcGGUCCgaaGCUCCGGGCUcuucgcucgACUCACu -3' miRNA: 3'- -GGaUCGGGug-CGAGGUUCGA---------UGAGUG- -5' |
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| 8104 | 3' | -54.6 | NC_001978.2 | + | 40969 | 0.74 | 0.248039 |
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Target: 5'- uUCUAGCgcugCCGCGC-CCAGcCUACUCACg -3' miRNA: 3'- -GGAUCG----GGUGCGaGGUUcGAUGAGUG- -5' |
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| 8104 | 3' | -54.6 | NC_001978.2 | + | 27589 | 0.74 | 0.248039 |
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Target: 5'- ---uGCCgaaGCGCccCCAAGCUACUCACg -3' miRNA: 3'- ggauCGGg--UGCGa-GGUUCGAUGAGUG- -5' |
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| 8104 | 3' | -54.6 | NC_001978.2 | + | 19257 | 1.12 | 0.000419 |
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Target: 5'- cCCUAGCCCACGCUCCAAGCUACUCACg -3' miRNA: 3'- -GGAUCGGGUGCGAGGUUCGAUGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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