miRNA: 3'- uCAU-CCGACUCGguaaAGCCG---------UCAGa -5'
miRNA: 3'- -UCAUCCGACUCGguaaaGCCGUCa-GA- -5'
miRNA: 3'- uCAUCCGaCUCG-GUaaaGCCGUCAGa -5'
miRNA: 3'- -UCAUCCGACUCGGUAAAGCCGUCAGA- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8106 | 3' | -53.4 | NC_001978.2 | + | 36536 | 0.66 | 0.719286 |
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Target: 5'- cGUAuGGCUGAGCgug-UCGGCucaugacugGGUCg -3' miRNA: 3'- uCAU-CCGACUCGguaaAGCCG---------UCAGa -5' |
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| 8106 | 3' | -53.4 | NC_001978.2 | + | 41037 | 0.73 | 0.295649 |
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Target: 5'- gAGUAGGCUGGGCg----CGGCAGcgCUa -3' miRNA: 3'- -UCAUCCGACUCGguaaaGCCGUCa-GA- -5' |
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| 8106 | 3' | -53.4 | NC_001978.2 | + | 37487 | 0.74 | 0.265892 |
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Target: 5'- cGUAGGCgGAGCgCAcucCGGCGGUCa -3' miRNA: 3'- uCAUCCGaCUCG-GUaaaGCCGUCAGa -5' |
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| 8106 | 3' | -53.4 | NC_001978.2 | + | 19799 | 1.08 | 0.001117 |
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Target: 5'- gAGUAGGCUGAGCCAUUUCGGCAGUCUu -3' miRNA: 3'- -UCAUCCGACUCGGUAAAGCCGUCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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