Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8108 | 5' | -56.3 | NC_001978.2 | + | 5279 | 0.67 | 0.487158 |
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Target: 5'- aCCgGCCUUCguGCCgUCgCGCUUCUCa-- -3' miRNA: 3'- aGG-CGGAAG--CGGaAG-GCGAAGAGcaa -5' |
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| 8108 | 5' | -56.3 | NC_001978.2 | + | 39903 | 0.67 | 0.476732 |
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Target: 5'- gUCCGUCguacgCGCCUgCCGCgcggUCUuCGUa -3' miRNA: 3'- -AGGCGGaa---GCGGAaGGCGa---AGA-GCAa -5' |
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| 8108 | 5' | -56.3 | NC_001978.2 | + | 21144 | 0.68 | 0.446142 |
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Target: 5'- cUCgGCCUUCGgCUcaaGCUUCUCGg- -3' miRNA: 3'- -AGgCGGAAGCgGAaggCGAAGAGCaa -5' |
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| 8108 | 5' | -56.3 | NC_001978.2 | + | 32065 | 0.68 | 0.436191 |
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Target: 5'- cUCCGCCUUCGUCU-CUGUggucaCUCGc- -3' miRNA: 3'- -AGGCGGAAGCGGAaGGCGaa---GAGCaa -5' |
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| 8108 | 5' | -56.3 | NC_001978.2 | + | 4585 | 0.7 | 0.327607 |
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Target: 5'- gUUCGuCCUUCaCCUUCCGCgugCUCGa- -3' miRNA: 3'- -AGGC-GGAAGcGGAAGGCGaa-GAGCaa -5' |
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| 8108 | 5' | -56.3 | NC_001978.2 | + | 23561 | 0.71 | 0.296144 |
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Target: 5'- cUUCGCCgcUGCCUUCCGCUcaUCGa- -3' miRNA: 3'- -AGGCGGaaGCGGAAGGCGAagAGCaa -5' |
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| 8108 | 5' | -56.3 | NC_001978.2 | + | 20440 | 0.71 | 0.288654 |
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Target: 5'- uUCgGCCUUCGCCgcuUCgGCUUC-CGg- -3' miRNA: 3'- -AGgCGGAAGCGGa--AGgCGAAGaGCaa -5' |
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| 8108 | 5' | -56.3 | NC_001978.2 | + | 8698 | 0.74 | 0.173389 |
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Target: 5'- gUCCGgCUUCGCCUUCCcCUUCagGUUc -3' miRNA: 3'- -AGGCgGAAGCGGAAGGcGAAGagCAA- -5' |
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| 8108 | 5' | -56.3 | NC_001978.2 | + | 21264 | 1.05 | 0.000955 |
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Target: 5'- cUCCGCCUUCGCCUUCCGCUUCUCGUUc -3' miRNA: 3'- -AGGCGGAAGCGGAAGGCGAAGAGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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