Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8109 | 5' | -58.6 | NC_001978.2 | + | 25468 | 0.66 | 0.478619 |
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Target: 5'- aCCGgUcgGCGGCGUaGUcGGCGGCAUc -3' miRNA: 3'- -GGCgAcaCGCCGCGaCA-CUGCCGUAc -5' |
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| 8109 | 5' | -58.6 | NC_001978.2 | + | 13199 | 0.66 | 0.467524 |
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Target: 5'- cCCGaCUGggGCGGCuuGCUGUggaccacGACGGCc-- -3' miRNA: 3'- -GGC-GACa-CGCCG--CGACA-------CUGCCGuac -5' |
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| 8109 | 5' | -58.6 | NC_001978.2 | + | 30217 | 0.66 | 0.458545 |
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Target: 5'- aCGCUGUGCGGgaagaGCgca-ACGGCAa- -3' miRNA: 3'- gGCGACACGCCg----CGacacUGCCGUac -5' |
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| 8109 | 5' | -58.6 | NC_001978.2 | + | 31313 | 0.67 | 0.392081 |
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Target: 5'- aCGUUG-GCGccgacGCGCUgagugGUGACGGUGUGg -3' miRNA: 3'- gGCGACaCGC-----CGCGA-----CACUGCCGUAC- -5' |
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| 8109 | 5' | -58.6 | NC_001978.2 | + | 23246 | 0.68 | 0.364716 |
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Target: 5'- uCCGU--UGCGGaCGCUGUGgcucagcucuucgGCGGCAc- -3' miRNA: 3'- -GGCGacACGCC-GCGACAC-------------UGCCGUac -5' |
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| 8109 | 5' | -58.6 | NC_001978.2 | + | 36418 | 0.68 | 0.357023 |
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Target: 5'- uUCGCUGUGC-GCGCUGacGAagGGCGUc -3' miRNA: 3'- -GGCGACACGcCGCGACa-CUg-CCGUAc -5' |
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| 8109 | 5' | -58.6 | NC_001978.2 | + | 10735 | 0.71 | 0.229744 |
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Target: 5'- uCCGCUGUGgGGCGUgccGcgaagggcgcuaucaACGGCGUGa -3' miRNA: 3'- -GGCGACACgCCGCGacaC---------------UGCCGUAC- -5' |
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| 8109 | 5' | -58.6 | NC_001978.2 | + | 2711 | 0.74 | 0.147783 |
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Target: 5'- cUCGCcGUucaCGGCGCUGUGAugUGGCGUGa -3' miRNA: 3'- -GGCGaCAc--GCCGCGACACU--GCCGUAC- -5' |
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| 8109 | 5' | -58.6 | NC_001978.2 | + | 21673 | 1.1 | 0.000284 |
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Target: 5'- uCCGCUGUGCGGCGCUGUGACGGCAUGa -3' miRNA: 3'- -GGCGACACGCCGCGACACUGCCGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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