miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
811 3' -48.4 NC_000866.4 + 70897 1.12 0.010927
Target:  5'- aAGGCCAUAUCUCAACCAUAUCCGAACg -3'
miRNA:   3'- -UCCGGUAUAGAGUUGGUAUAGGCUUG- -5'
811 3' -48.4 NC_000866.4 + 94209 0.76 0.8122
Target:  5'- uAGGUCGUGUUgUCGGCCAagAUCCGAAUg -3'
miRNA:   3'- -UCCGGUAUAG-AGUUGGUa-UAGGCUUG- -5'
811 3' -48.4 NC_000866.4 + 32466 0.66 0.999551
Target:  5'- uGGCCAg----CAGCCG-AUCCGAGu -3'
miRNA:   3'- uCCGGUauagaGUUGGUaUAGGCUUg -5'
811 3' -48.4 NC_000866.4 + 62349 0.66 0.999303
Target:  5'- uGGCCA--UUUCAAUgAUGgcacauagcUCCGAGCa -3'
miRNA:   3'- uCCGGUauAGAGUUGgUAU---------AGGCUUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.