Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8110 | 5' | -55.4 | NC_001978.2 | + | 2605 | 0.66 | 0.582805 |
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Target: 5'- cGGCAcGCGUCGUacacgGCCugagUGGCa- -3' miRNA: 3'- -CCGUuCGCAGCGa----CGGuagaACCGaa -5' |
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| 8110 | 5' | -55.4 | NC_001978.2 | + | 22043 | 0.66 | 0.571473 |
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Target: 5'- cGCGGucGCG-CGCUGCCAUgUcGGCa- -3' miRNA: 3'- cCGUU--CGCaGCGACGGUAgAaCCGaa -5' |
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| 8110 | 5' | -55.4 | NC_001978.2 | + | 33717 | 0.67 | 0.483436 |
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Target: 5'- cGGCugAAGCGUCGUcgucuUGCCGUUguacaugGGCa- -3' miRNA: 3'- -CCG--UUCGCAGCG-----ACGGUAGaa-----CCGaa -5' |
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| 8110 | 5' | -55.4 | NC_001978.2 | + | 11751 | 0.68 | 0.452093 |
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Target: 5'- gGGCGAGCcgcaaCGCUGCCGUaCUgagugaagcGGCUUa -3' miRNA: 3'- -CCGUUCGca---GCGACGGUA-GAa--------CCGAA- -5' |
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| 8110 | 5' | -55.4 | NC_001978.2 | + | 37512 | 0.7 | 0.365307 |
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Target: 5'- cGCGAGCGUCGuCUGUCuUCgucGGCg- -3' miRNA: 3'- cCGUUCGCAGC-GACGGuAGaa-CCGaa -5' |
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| 8110 | 5' | -55.4 | NC_001978.2 | + | 14559 | 0.71 | 0.293772 |
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Target: 5'- cGCGAGaaCGUCGCUGCCccugacgguggcgcUCUUGGCa- -3' miRNA: 3'- cCGUUC--GCAGCGACGGu-------------AGAACCGaa -5' |
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| 8110 | 5' | -55.4 | NC_001978.2 | + | 13107 | 0.72 | 0.274409 |
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Target: 5'- gGGCAA-CGUCGCgauaacgucacCCGUCUUGGCUUc -3' miRNA: 3'- -CCGUUcGCAGCGac---------GGUAGAACCGAA- -5' |
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| 8110 | 5' | -55.4 | NC_001978.2 | + | 22738 | 0.76 | 0.14591 |
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Target: 5'- cGGCGucguguuCGUCGCUGCCGUCgucgGGCUc -3' miRNA: 3'- -CCGUuc-----GCAGCGACGGUAGaa--CCGAa -5' |
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| 8110 | 5' | -55.4 | NC_001978.2 | + | 21998 | 1.08 | 0.000563 |
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Target: 5'- cGGCAAGCGUCGCUGCCAUCUUGGCUUg -3' miRNA: 3'- -CCGUUCGCAGCGACGGUAGAACCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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