Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8112 | 3' | -53.7 | NC_001978.2 | + | 15726 | 0.66 | 0.76193 |
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Target: 5'- gGUCacuaUGu-GGGCGAaGUCGGGcgGCGu -3' miRNA: 3'- gUAG----ACuuCCCGCUgCAGCCCuaUGC- -5' |
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| 8112 | 3' | -53.7 | NC_001978.2 | + | 31728 | 0.66 | 0.76193 |
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Target: 5'- uGUCauaAAGGGCGuCGUCggcgaaagggcuGGGGUGCGu -3' miRNA: 3'- gUAGac-UUCCCGCuGCAG------------CCCUAUGC- -5' |
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| 8112 | 3' | -53.7 | NC_001978.2 | + | 3768 | 0.66 | 0.729989 |
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Target: 5'- gGUCUGAAggaacuGGGCuuCGUCGGGGc--- -3' miRNA: 3'- gUAGACUU------CCCGcuGCAGCCCUaugc -5' |
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| 8112 | 3' | -53.7 | NC_001978.2 | + | 16038 | 0.69 | 0.540769 |
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Target: 5'- gGUCcGAAGGGUGACc-CGGG-UACGg -3' miRNA: 3'- gUAGaCUUCCCGCUGcaGCCCuAUGC- -5' |
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| 8112 | 3' | -53.7 | NC_001978.2 | + | 15550 | 0.7 | 0.529871 |
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Target: 5'- aGUCgggcgGcAGGGGCGACGUCGGcaaGGUcgACGg -3' miRNA: 3'- gUAGa----C-UUCCCGCUGCAGCC---CUA--UGC- -5' |
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| 8112 | 3' | -53.7 | NC_001978.2 | + | 29618 | 0.83 | 0.073212 |
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Target: 5'- aCAUCUGAAGGaUGACGUCGGGuUACGc -3' miRNA: 3'- -GUAGACUUCCcGCUGCAGCCCuAUGC- -5' |
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| 8112 | 3' | -53.7 | NC_001978.2 | + | 22415 | 1.08 | 0.001338 |
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Target: 5'- uCAUCUGAAGGGCGACGUCGGGAUACGu -3' miRNA: 3'- -GUAGACUUCCCGCUGCAGCCCUAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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