Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8114 | 5' | -52.3 | NC_001978.2 | + | 9819 | 0.66 | 0.834865 |
Target: 5'- aGGUCGAcg-GCGCgGCgaaGUCG-GCa -3' miRNA: 3'- gCCAGCUcuaUGCGgUGaa-CAGCuCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 8258 | 0.66 | 0.824647 |
Target: 5'- aCGGcgcccuUCGcGG-ACGCCgugaccuuaauggACUUGUCGAGCu -3' miRNA: 3'- -GCC------AGCuCUaUGCGG-------------UGAACAGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 14154 | 0.66 | 0.807372 |
Target: 5'- cCGGUCGAGuggucggcuaucggGCGCgGCauggUGUCcGGCa -3' miRNA: 3'- -GCCAGCUCua------------UGCGgUGa---ACAGcUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 33820 | 0.67 | 0.765774 |
Target: 5'- -uGUCGAcgugACGUCGCUcgacUGUCGGGCc -3' miRNA: 3'- gcCAGCUcua-UGCGGUGA----ACAGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 7562 | 0.67 | 0.744541 |
Target: 5'- uGGUCGAGuucgACGCgACgucgcUUGAGCu -3' miRNA: 3'- gCCAGCUCua--UGCGgUGaac--AGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 430 | 0.68 | 0.733737 |
Target: 5'- cCGGUCGcgcacccGAUGCuUCGCUaUGUCGAGUc -3' miRNA: 3'- -GCCAGCu------CUAUGcGGUGA-ACAGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 32443 | 0.68 | 0.733737 |
Target: 5'- aGGUUGuG-UGCGCC-CUUccaugcGUCGAGCc -3' miRNA: 3'- gCCAGCuCuAUGCGGuGAA------CAGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 33916 | 0.68 | 0.722825 |
Target: 5'- aCGGUCGgcacccGGGUaACGCCGuCggcccgacaGUCGAGCg -3' miRNA: 3'- -GCCAGC------UCUA-UGCGGU-Gaa-------CAGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 39714 | 0.68 | 0.689565 |
Target: 5'- -uGUUGaAGAUGCGUUccgcaacgaACUUGUCGAGUg -3' miRNA: 3'- gcCAGC-UCUAUGCGG---------UGAACAGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 24776 | 0.69 | 0.667077 |
Target: 5'- aGcGUCGGGcgACGgCAUacgggGUCGAGCa -3' miRNA: 3'- gC-CAGCUCuaUGCgGUGaa---CAGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 9653 | 0.69 | 0.633113 |
Target: 5'- cCGGUCGcGAaggACGCCcuagacaagacGCUUGaCGGGCu -3' miRNA: 3'- -GCCAGCuCUa--UGCGG-----------UGAACaGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 5373 | 0.7 | 0.576622 |
Target: 5'- gGGUCGGGcgAcCGCCGggaUUGUCG-GCg -3' miRNA: 3'- gCCAGCUCuaU-GCGGUg--AACAGCuCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 6412 | 0.71 | 0.554299 |
Target: 5'- cCGaUCGGGaAUGCGCgaCACgugGUCGAGCa -3' miRNA: 3'- -GCcAGCUC-UAUGCG--GUGaa-CAGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 39055 | 0.71 | 0.543231 |
Target: 5'- uGGUCGAG-UGCGCaaGCUUGUUGAu- -3' miRNA: 3'- gCCAGCUCuAUGCGg-UGAACAGCUcg -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 29319 | 0.72 | 0.499769 |
Target: 5'- -aGUCGAGAagcccaGCGUCACgcaUGUUGAGCa -3' miRNA: 3'- gcCAGCUCUa-----UGCGGUGa--ACAGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 37822 | 0.73 | 0.437705 |
Target: 5'- gGGUUGAGGgguCGCCGuc-GUCGGGCa -3' miRNA: 3'- gCCAGCUCUau-GCGGUgaaCAGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 23865 | 0.74 | 0.389514 |
Target: 5'- gGGUCGAGcgcUGCCACUaGUCG-GCa -3' miRNA: 3'- gCCAGCUCuauGCGGUGAaCAGCuCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 32529 | 0.74 | 0.388586 |
Target: 5'- uCGGUCGAGucgGCaccgugaGCCACUgggCGAGCg -3' miRNA: 3'- -GCCAGCUCua-UG-------CGGUGAacaGCUCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 36885 | 0.75 | 0.311987 |
Target: 5'- cCGGUUGAGGUcGCuGCCGCUgccgGUCG-GCa -3' miRNA: 3'- -GCCAGCUCUA-UG-CGGUGAa---CAGCuCG- -5' |
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8114 | 5' | -52.3 | NC_001978.2 | + | 11524 | 0.75 | 0.311987 |
Target: 5'- aGGUCGAGcgGCGCCgaccGCUUG-CGuGCc -3' miRNA: 3'- gCCAGCUCuaUGCGG----UGAACaGCuCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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