Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8123 | 3' | -50.4 | NC_001978.2 | + | 32160 | 0.68 | 0.786291 |
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Target: 5'- gUCAUCACgGCACGGUcGGCCgGAAcGGCa -3' miRNA: 3'- -AGUGGUG-UGUGCUA-CUGGaCUU-UCGg -5' |
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| 8123 | 3' | -50.4 | NC_001978.2 | + | 33617 | 0.7 | 0.6641 |
|
Target: 5'- gUC-CCACA-GCGAaaUGACCUGGGuGCCc -3' miRNA: 3'- -AGuGGUGUgUGCU--ACUGGACUUuCGG- -5' |
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| 8123 | 3' | -50.4 | NC_001978.2 | + | 35104 | 0.68 | 0.775808 |
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Target: 5'- -aGCCACGCGCGGgcaGcACCUGAcGGGaCCc -3' miRNA: 3'- agUGGUGUGUGCUa--C-UGGACU-UUC-GG- -5' |
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| 8123 | 3' | -50.4 | NC_001978.2 | + | 36097 | 0.67 | 0.854004 |
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Target: 5'- aCACCuggGCGCGCGAggaaGCC--GAAGCCg -3' miRNA: 3'- aGUGG---UGUGUGCUac--UGGacUUUCGG- -5' |
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| 8123 | 3' | -50.4 | NC_001978.2 | + | 37763 | 0.66 | 0.887272 |
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Target: 5'- aUCACCcagggGCGCACGAcgGGCg-GGuuGCCg -3' miRNA: 3'- -AGUGG-----UGUGUGCUa-CUGgaCUuuCGG- -5' |
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| 8123 | 3' | -50.4 | NC_001978.2 | + | 37856 | 0.74 | 0.439489 |
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Target: 5'- gCGCCAUGCugGGUGACCcauucgagcggcaUGAGGGUUg -3' miRNA: 3'- aGUGGUGUGugCUACUGG-------------ACUUUCGG- -5' |
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| 8123 | 3' | -50.4 | NC_001978.2 | + | 39272 | 0.69 | 0.75436 |
|
Target: 5'- uUCGCCGCugA-GGUGAUCUacaaGAagAAGCCg -3' miRNA: 3'- -AGUGGUGugUgCUACUGGA----CU--UUCGG- -5' |
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| 8123 | 3' | -50.4 | NC_001978.2 | + | 40366 | 0.7 | 0.709909 |
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Target: 5'- gUCACCACAgcacaGCGGggcgagccguUGACCUGggGGa- -3' miRNA: 3'- -AGUGGUGUg----UGCU----------ACUGGACuuUCgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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