Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8126 | 3' | -54.5 | NC_001978.2 | + | 6390 | 0.66 | 0.666877 |
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Target: 5'- uGGUcGAGCAcguauUGUCGCgCga-GUGUGCGc -3' miRNA: 3'- -UCA-CUCGU-----ACAGCGgGaaaCGCACGC- -5' |
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| 8126 | 3' | -54.5 | NC_001978.2 | + | 11023 | 0.66 | 0.632765 |
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Target: 5'- -uUGGGCGcgaaGUCGCCUgg-GCGcUGCGg -3' miRNA: 3'- ucACUCGUa---CAGCGGGaaaCGC-ACGC- -5' |
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| 8126 | 3' | -54.5 | NC_001978.2 | + | 28750 | 0.66 | 0.632765 |
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Target: 5'- -aUGGaCAUG-CGCCCcgUGCGUGCc -3' miRNA: 3'- ucACUcGUACaGCGGGaaACGCACGc -5' |
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| 8126 | 3' | -54.5 | NC_001978.2 | + | 30927 | 0.66 | 0.621379 |
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Target: 5'- gGGUGcgacaugaaGGCAUGUgCGCCCaggucgGCGUGaCGu -3' miRNA: 3'- -UCAC---------UCGUACA-GCGGGaaa---CGCAC-GC- -5' |
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| 8126 | 3' | -54.5 | NC_001978.2 | + | 24597 | 0.68 | 0.554736 |
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Target: 5'- uGUGucGGCGUGcUUGCCCUUgccauuaccgcaaugGUGUGCGc -3' miRNA: 3'- uCAC--UCGUAC-AGCGGGAAa--------------CGCACGC- -5' |
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| 8126 | 3' | -54.5 | NC_001978.2 | + | 18390 | 0.68 | 0.543618 |
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Target: 5'- gGGUGAGCcu-UCGCCCaacucaacgcggcGCGUGCGu -3' miRNA: 3'- -UCACUCGuacAGCGGGaaa----------CGCACGC- -5' |
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| 8126 | 3' | -54.5 | NC_001978.2 | + | 22103 | 0.68 | 0.509655 |
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Target: 5'- gGGUGAGUAcGUCGgCCgugUGCGcGCu -3' miRNA: 3'- -UCACUCGUaCAGCgGGaa-ACGCaCGc -5' |
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| 8126 | 3' | -54.5 | NC_001978.2 | + | 1055 | 0.69 | 0.456896 |
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Target: 5'- --cGAGCGUGUaguaaCCCg--GCGUGCGg -3' miRNA: 3'- ucaCUCGUACAgc---GGGaaaCGCACGC- -5' |
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| 8126 | 3' | -54.5 | NC_001978.2 | + | 26567 | 0.93 | 0.011192 |
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Target: 5'- cAGUGAGCAUGUCGCCCUUgucgUGCGcGCGg -3' miRNA: 3'- -UCACUCGUACAGCGGGAA----ACGCaCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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