Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8126 | 5' | -56 | NC_001978.2 | + | 39645 | 0.72 | 0.295441 |
Target: 5'- gCCCagGUGCGGacGGGUCGACCACcuuCCGg- -3' miRNA: 3'- -GGG--CAUGCU--UCCAGCUGGUGc--GGCaa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 10836 | 0.72 | 0.295441 |
Target: 5'- gCCCaGUucaGAAGaUCGAUCACGCCGUUg -3' miRNA: 3'- -GGG-CAug-CUUCcAGCUGGUGCGGCAA- -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 210 | 0.77 | 0.144013 |
Target: 5'- cCCCGUcguguACGAGGGUCGAgCGCcCCGUg -3' miRNA: 3'- -GGGCA-----UGCUUCCAGCUgGUGcGGCAa -5' |
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8126 | 5' | -56 | NC_001978.2 | + | 26530 | 0.98 | 0.004334 |
Target: 5'- gCCCGUACG-AGGUCGACCACGCCGUUc -3' miRNA: 3'- -GGGCAUGCuUCCAGCUGGUGCGGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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