Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8127 | 5' | -61.3 | NC_001978.2 | + | 2794 | 0.7 | 0.16074 |
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Target: 5'- aUGCCgUUGUCUucacgccacauCACAGCGCCGUGAAc -3' miRNA: 3'- cGCGGgAACGGG-----------GUGUCGUGGCGCUU- -5' |
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| 8127 | 5' | -61.3 | NC_001978.2 | + | 35276 | 0.7 | 0.156415 |
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Target: 5'- gGCGCCCgUGUCCUucCGGCGCC-CGAu -3' miRNA: 3'- -CGCGGGaACGGGGu-GUCGUGGcGCUu -5' |
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| 8127 | 5' | -61.3 | NC_001978.2 | + | 1084 | 0.71 | 0.136331 |
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Target: 5'- -aGCUCUUcGCCCCGgAGCACaaCGCGGAc -3' miRNA: 3'- cgCGGGAA-CGGGGUgUCGUG--GCGCUU- -5' |
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| 8127 | 5' | -61.3 | NC_001978.2 | + | 21548 | 0.71 | 0.136331 |
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Target: 5'- aGCGCCCgucuccGCCCCcaACAGCcCCGuCGGGu -3' miRNA: 3'- -CGCGGGaa----CGGGG--UGUCGuGGC-GCUU- -5' |
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| 8127 | 5' | -61.3 | NC_001978.2 | + | 36665 | 0.71 | 0.13261 |
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Target: 5'- -aGCCCUgagcGCCCCugAGCcggUCGCGGAa -3' miRNA: 3'- cgCGGGAa---CGGGGugUCGu--GGCGCUU- -5' |
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| 8127 | 5' | -61.3 | NC_001978.2 | + | 24610 | 0.74 | 0.087 |
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Target: 5'- uUGCCCUUGCCauuaCCGCAauggugugcgcGCACCGUGAGc -3' miRNA: 3'- cGCGGGAACGG----GGUGU-----------CGUGGCGCUU- -5' |
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| 8127 | 5' | -61.3 | NC_001978.2 | + | 10804 | 0.75 | 0.067255 |
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Target: 5'- aGCGCCCUUcgcggcacGCCCCACAGCGgaagcaaGCGAc -3' miRNA: 3'- -CGCGGGAA--------CGGGGUGUCGUgg-----CGCUu -5' |
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| 8127 | 5' | -61.3 | NC_001978.2 | + | 26743 | 1.07 | 0.000197 |
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Target: 5'- gGCGCCCUUGCCCCACAGCACCGCGAAa -3' miRNA: 3'- -CGCGGGAACGGGGUGUCGUGGCGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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