Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8134 | 5' | -55.7 | NC_001978.2 | + | 4479 | 0.66 | 0.624875 |
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Target: 5'- uGGuuGaACGUGGCgUacacgucguucUGUCGcAGCGACg -3' miRNA: 3'- gUCggC-UGCACUGgA-----------ACAGC-UCGCUG- -5' |
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| 8134 | 5' | -55.7 | NC_001978.2 | + | 7305 | 0.66 | 0.629349 |
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Target: 5'- -cGCCGcccgugucaguaACgGUGACCUUGUCGAacgccgccgucagaaGCGAa -3' miRNA: 3'- guCGGC------------UG-CACUGGAACAGCU---------------CGCUg -5' |
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| 8134 | 5' | -55.7 | NC_001978.2 | + | 8246 | 0.66 | 0.634942 |
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Target: 5'- gCGGaCGcCGUGACCUuaauggacuUGUCGAGCuugcccgGACg -3' miRNA: 3'- -GUCgGCuGCACUGGA---------ACAGCUCG-------CUG- -5' |
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| 8134 | 5' | -55.7 | NC_001978.2 | + | 33836 | 0.66 | 0.636061 |
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Target: 5'- cCAGCaCGcuucGCGgaacGCCUUcacgGUCGAGCGACc -3' miRNA: 3'- -GUCG-GC----UGCac--UGGAA----CAGCUCGCUG- -5' |
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| 8134 | 5' | -55.7 | NC_001978.2 | + | 35918 | 0.66 | 0.636061 |
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Target: 5'- aAGCCGACGcuaGAcgcCCUUGaUCGcugaaGGCGGCu -3' miRNA: 3'- gUCGGCUGCa--CU---GGAAC-AGC-----UCGCUG- -5' |
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| 8134 | 5' | -55.7 | NC_001978.2 | + | 9508 | 0.66 | 0.636061 |
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Target: 5'- uGGCgcucgCGACGcUGACCUUGUCGc-UGACu -3' miRNA: 3'- gUCG-----GCUGC-ACUGGAACAGCucGCUG- -5' |
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| 8134 | 5' | -55.7 | NC_001978.2 | + | 21089 | 0.66 | 0.636061 |
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Target: 5'- gAGCCGAa--GGCCgaGaaGAGCGACg -3' miRNA: 3'- gUCGGCUgcaCUGGaaCagCUCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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