miRNA: 3'- aCGUAGaccuuCAUUGGUUAGUCUUA- -5'
miRNA: 3'- acGUAGACCUUCAU--UGGUUAGU-CUua -5'
miRNA: 3'- -ACGUAGACCUUCaUUGGuuaGUCUUA- -5'
miRNA: 3'- -ACGUAGACCUUCAUUGGUUAGUCUUA- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 814 | 3' | -46.1 | NC_000866.4 | + | 167854 | 0.68 | 0.99941 |
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Target: 5'- aGCAUCguu--GUAAUCAAUCGGAAUu -3' miRNA: 3'- aCGUAGaccuuCAUUGGUUAGUCUUA- -5' |
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| 814 | 3' | -46.1 | NC_000866.4 | + | 125404 | 0.69 | 0.998323 |
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Target: 5'- -aCAUCUGGAGGUGauACUAAUCGuGAu- -3' miRNA: 3'- acGUAGACCUUCAU--UGGUUAGU-CUua -5' |
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| 814 | 3' | -46.1 | NC_000866.4 | + | 137419 | 0.75 | 0.950201 |
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Target: 5'- gUGCAUCUGGAAG-AACUua-CAGAAUu -3' miRNA: 3'- -ACGUAGACCUUCaUUGGuuaGUCUUA- -5' |
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| 814 | 3' | -46.1 | NC_000866.4 | + | 137177 | 1.08 | 0.028868 |
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Target: 5'- gUGCAUCUGGAAGUAACCAAUCAGAAUa -3' miRNA: 3'- -ACGUAGACCUUCAUUGGUUAGUCUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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