Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8142 | 5' | -56.8 | NC_001978.2 | + | 26121 | 0.66 | 0.564665 |
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Target: 5'- ---gCGCGACUUCG-GCCUuacgguGCUCCGCc -3' miRNA: 3'- cagaGCGCUGAGGCaUGGG------UGAGGUG- -5' |
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| 8142 | 5' | -56.8 | NC_001978.2 | + | 8229 | 0.66 | 0.521435 |
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Target: 5'- -gCUCaGCGGCUCgCGUGCCgUAUgccgCCGCa -3' miRNA: 3'- caGAG-CGCUGAG-GCAUGG-GUGa---GGUG- -5' |
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| 8142 | 5' | -56.8 | NC_001978.2 | + | 35752 | 0.67 | 0.5003 |
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Target: 5'- cGUCUC-CGGCUCCGUACgggGCUUCGa -3' miRNA: 3'- -CAGAGcGCUGAGGCAUGgg-UGAGGUg -5' |
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| 8142 | 5' | -56.8 | NC_001978.2 | + | 6177 | 0.67 | 0.479552 |
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Target: 5'- gGUCUUGaCGACUCCGcGCUUuuCUCCGa -3' miRNA: 3'- -CAGAGC-GCUGAGGCaUGGGu-GAGGUg -5' |
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| 8142 | 5' | -56.8 | NC_001978.2 | + | 34338 | 0.67 | 0.469337 |
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Target: 5'- cGUCguguacagCGUGGCUCCG-GCaaGCUCCGCc -3' miRNA: 3'- -CAGa-------GCGCUGAGGCaUGggUGAGGUG- -5' |
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| 8142 | 5' | -56.8 | NC_001978.2 | + | 36783 | 0.73 | 0.197534 |
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Target: 5'- cGUCUC-CGGCUCCGUgacggGCUCagGCUCCGCc -3' miRNA: 3'- -CAGAGcGCUGAGGCA-----UGGG--UGAGGUG- -5' |
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| 8142 | 5' | -56.8 | NC_001978.2 | + | 35315 | 1.1 | 0.00041 |
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Target: 5'- cGUCUCGCGACUCCGUACCCACUCCACa -3' miRNA: 3'- -CAGAGCGCUGAGGCAUGGGUGAGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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