miRNA: 3'- -UUCCCGCAGca--GCAGUa----CuCCGACAU- -5'
miRNA: 3'- -UUCCCGCAGCAGcAGUACUcCGacau -5'
miRNA: 3'- uucCCGCAGCAGCAGUACU--CCGacau -5'
miRNA: 3'- -UUCCCGCAGCAGCaguac-UCCGACau -5'
miRNA: 3'- -UUCCCGCAGCAGCAGUACUCCGACAU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8144 | 3' | -56.5 | NC_001978.2 | + | 35980 | 0.66 | 0.543231 |
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Target: 5'- cAAGGGCGUCucagCGUCGgcuucGuGGUUGUGc -3' miRNA: 3'- -UUCCCGCAGca--GCAGUa----CuCCGACAU- -5' |
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| 8144 | 3' | -56.5 | NC_001978.2 | + | 26812 | 0.71 | 0.274241 |
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Target: 5'- gAAGGGCGUCGUCuUCGUGAuGCg--- -3' miRNA: 3'- -UUCCCGCAGCAGcAGUACUcCGacau -5' |
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| 8144 | 3' | -56.5 | NC_001978.2 | + | 5202 | 0.72 | 0.22158 |
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Target: 5'- --cGGCGUCGUCGUCGUGGucGGCg--- -3' miRNA: 3'- uucCCGCAGCAGCAGUACU--CCGacau -5' |
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| 8144 | 3' | -56.5 | NC_001978.2 | + | 31722 | 0.74 | 0.168102 |
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Target: 5'- aAAGGGCGUCGUCGgcgaaaGGGCUGg- -3' miRNA: 3'- -UUCCCGCAGCAGCaguac-UCCGACau -5' |
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| 8144 | 3' | -56.5 | NC_001978.2 | + | 36400 | 1.05 | 0.000862 |
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Target: 5'- gAAGGGCGUCGUCGUCAUGAGGCUGUAg -3' miRNA: 3'- -UUCCCGCAGCAGCAGUACUCCGACAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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