Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8146 | 5' | -57.5 | NC_001978.2 | + | 36136 | 0.66 | 0.505969 |
Target: 5'- gGCGgGugaguGGCCCGugUagcacgUCGGCGAGuAGGg -3' miRNA: 3'- aUGUgC-----CCGGGCugA------AGCCGCUC-UUC- -5' |
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8146 | 5' | -57.5 | NC_001978.2 | + | 12559 | 0.67 | 0.46476 |
Target: 5'- gACAUGGGUgcggcUCGACUcCGGCGAuugguucacGAAGg -3' miRNA: 3'- aUGUGCCCG-----GGCUGAaGCCGCU---------CUUC- -5' |
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8146 | 5' | -57.5 | NC_001978.2 | + | 27414 | 0.67 | 0.46476 |
Target: 5'- aACACGGGUauugCG-CUugUCGGCGuGAAGu -3' miRNA: 3'- aUGUGCCCGg---GCuGA--AGCCGCuCUUC- -5' |
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8146 | 5' | -57.5 | NC_001978.2 | + | 36074 | 0.67 | 0.444819 |
Target: 5'- cUACACGGGCcacucacccgCCGACaccugggCGcGCGAGGAa -3' miRNA: 3'- -AUGUGCCCG----------GGCUGaa-----GC-CGCUCUUc -5' |
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8146 | 5' | -57.5 | NC_001978.2 | + | 2976 | 0.67 | 0.444819 |
Target: 5'- uUACAaccUGGGCgCCGuucgGCUUcCGGCGAGAc- -3' miRNA: 3'- -AUGU---GCCCG-GGC----UGAA-GCCGCUCUuc -5' |
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8146 | 5' | -57.5 | NC_001978.2 | + | 37748 | 0.68 | 0.397164 |
Target: 5'- uUGCGCGcuGCCCGACgaCGGCGAc--- -3' miRNA: 3'- -AUGUGCc-CGGGCUGaaGCCGCUcuuc -5' |
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8146 | 5' | -57.5 | NC_001978.2 | + | 23776 | 0.68 | 0.379042 |
Target: 5'- cGCAuCGGGCCaCG-CUUCGGCGuGu-- -3' miRNA: 3'- aUGU-GCCCGG-GCuGAAGCCGCuCuuc -5' |
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8146 | 5' | -57.5 | NC_001978.2 | + | 4054 | 0.69 | 0.344498 |
Target: 5'- gACGCcccuGCCCGACggauUgGGCGAGAAGu -3' miRNA: 3'- aUGUGcc--CGGGCUGa---AgCCGCUCUUC- -5' |
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8146 | 5' | -57.5 | NC_001978.2 | + | 5369 | 0.7 | 0.278111 |
Target: 5'- -uCACGGGUCgGGCgaccgccgggauugUCGGCGAGAc- -3' miRNA: 3'- auGUGCCCGGgCUGa-------------AGCCGCUCUuc -5' |
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8146 | 5' | -57.5 | NC_001978.2 | + | 36675 | 1.06 | 0.000686 |
Target: 5'- gUACACGGGCCCGACUUCGGCGAGAAGc -3' miRNA: 3'- -AUGUGCCCGGGCUGAAGCCGCUCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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