Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8150 | 5' | -49.7 | NC_001978.2 | + | 32017 | 0.66 | 0.89902 |
Target: 5'- aGGCGGAGcUUCaggcUGagcgGgGGCGCGUCg -3' miRNA: 3'- -UCGCUUCuAAGaa--ACa---CgCUGCGCAG- -5' |
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8150 | 5' | -49.7 | NC_001978.2 | + | 26504 | 0.66 | 0.894533 |
Target: 5'- cGCGAAGcucaacagGUGCGcguuCGCGUCg -3' miRNA: 3'- uCGCUUCuaagaaa-CACGCu---GCGCAG- -5' |
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8150 | 5' | -49.7 | NC_001978.2 | + | 3581 | 0.67 | 0.883646 |
Target: 5'- gAGCGAAGAgggUUUggcccGUGCG-CGCGa- -3' miRNA: 3'- -UCGCUUCUa--AGAaa---CACGCuGCGCag -5' |
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8150 | 5' | -49.7 | NC_001978.2 | + | 22969 | 0.69 | 0.747483 |
Target: 5'- gGGCGcuGAGGgggUUUUUGUGUuGCGCGUUg -3' miRNA: 3'- -UCGC--UUCUa--AGAAACACGcUGCGCAG- -5' |
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8150 | 5' | -49.7 | NC_001978.2 | + | 17741 | 0.71 | 0.643993 |
Target: 5'- cGCGAAGGgcUUCg----GCGACGCGUa -3' miRNA: 3'- uCGCUUCU--AAGaaacaCGCUGCGCAg -5' |
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8150 | 5' | -49.7 | NC_001978.2 | + | 7518 | 0.77 | 0.353688 |
Target: 5'- cAGcCGGAGug-CUUgUGUGCGugGCGUCa -3' miRNA: 3'- -UC-GCUUCuaaGAA-ACACGCugCGCAG- -5' |
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8150 | 5' | -49.7 | NC_001978.2 | + | 38892 | 1.11 | 0.001749 |
Target: 5'- cAGCGAAGAUUCUUUGUGCGACGCGUCg -3' miRNA: 3'- -UCGCUUCUAAGAAACACGCUGCGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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