Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8151 | 5' | -60.2 | NC_001978.2 | + | 11400 | 0.66 | 0.312276 |
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Target: 5'- --cCGGUU-CCGuuUCCCGGGCGCa- -3' miRNA: 3'- auuGCCAAcGGCccAGGGUCCGCGaa -5' |
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| 8151 | 5' | -60.2 | NC_001978.2 | + | 771 | 0.67 | 0.304586 |
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Target: 5'- aGACGGUcaagguucaCCGGGaCCCgaAGGCGCUg -3' miRNA: 3'- aUUGCCAac-------GGCCCaGGG--UCCGCGAa -5' |
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| 8151 | 5' | -60.2 | NC_001978.2 | + | 10960 | 0.68 | 0.229498 |
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Target: 5'- -cAUGcGUUGUCGGGUUCCGcGGCGUg- -3' miRNA: 3'- auUGC-CAACGGCCCAGGGU-CCGCGaa -5' |
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| 8151 | 5' | -60.2 | NC_001978.2 | + | 23594 | 0.69 | 0.217652 |
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Target: 5'- -cACGGUgauUGCCGGGUUC--GGCGCg- -3' miRNA: 3'- auUGCCA---ACGGCCCAGGguCCGCGaa -5' |
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| 8151 | 5' | -60.2 | NC_001978.2 | + | 15515 | 0.7 | 0.180246 |
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Target: 5'- cGACGuacGUUGCCgucccGGGUCCCAGcGCGUa- -3' miRNA: 3'- aUUGC---CAACGG-----CCCAGGGUC-CGCGaa -5' |
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| 8151 | 5' | -60.2 | NC_001978.2 | + | 39281 | 1.04 | 0.000411 |
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Target: 5'- aUAACGGUUGCCGGGUCCCAGGCGCUUg -3' miRNA: 3'- -AUUGCCAACGGCCCAGGGUCCGCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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