Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
8152 | 3' | -58.6 | NC_001978.2 | + | 38186 | 0.66 | 0.426454 |
Target: 5'- uGCCGGa-GCaauCGCCCUGGGuGGGu- -3' miRNA: 3'- -CGGCCagCGau-GUGGGACCCuCUCua -5' |
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8152 | 3' | -58.6 | NC_001978.2 | + | 30687 | 0.67 | 0.407566 |
Target: 5'- cGCCGGagUCGUcuCGCCCUGGGuGGc-- -3' miRNA: 3'- -CGGCC--AGCGauGUGGGACCCuCUcua -5' |
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8152 | 3' | -58.6 | NC_001978.2 | + | 34876 | 0.67 | 0.398321 |
Target: 5'- cGCCGGUUG-UACucCCCaGGGAuGAGAc -3' miRNA: 3'- -CGGCCAGCgAUGu-GGGaCCCU-CUCUa -5' |
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8152 | 3' | -58.6 | NC_001978.2 | + | 25468 | 0.67 | 0.394661 |
Target: 5'- aCCGGUCgGCggcguagucggcgGCAUCCUGGGuGAGc- -3' miRNA: 3'- cGGCCAG-CGa------------UGUGGGACCCuCUCua -5' |
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8152 | 3' | -58.6 | NC_001978.2 | + | 11472 | 0.69 | 0.290967 |
Target: 5'- gGUCGGaCGCUGCGCCC-GGGAaacGGAa -3' miRNA: 3'- -CGGCCaGCGAUGUGGGaCCCUc--UCUa -5' |
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8152 | 3' | -58.6 | NC_001978.2 | + | 32653 | 0.71 | 0.218966 |
Target: 5'- gGCCGacugaCGCUgACGCCCUGGGGGAa-- -3' miRNA: 3'- -CGGCca---GCGA-UGUGGGACCCUCUcua -5' |
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8152 | 3' | -58.6 | NC_001978.2 | + | 40699 | 0.94 | 0.004156 |
Target: 5'- cGCCGGUCGCUcaACCCUGGGAGAGAUc -3' miRNA: 3'- -CGGCCAGCGAugUGGGACCCUCUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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