Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8152 | 5' | -59.5 | NC_001978.2 | + | 40879 | 0.66 | 0.430369 |
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Target: 5'- aGGCaaGCCUucGGGuUGGGUGcGUGCUCGa -3' miRNA: 3'- aCUGgcCGGA--CUC-ACCCAU-CGCGAGC- -5' |
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| 8152 | 5' | -59.5 | NC_001978.2 | + | 2595 | 0.66 | 0.411544 |
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Target: 5'- cGuACaCGGCCUGAGUGGcaggcaccuGCGCgCGa -3' miRNA: 3'- aC-UG-GCCGGACUCACCcau------CGCGaGC- -5' |
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| 8152 | 5' | -59.5 | NC_001978.2 | + | 5098 | 0.69 | 0.280648 |
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Target: 5'- cUGuCCGGCCUucaGGGUucGGGcuccGGCGCUCa -3' miRNA: 3'- -ACuGGCCGGA---CUCA--CCCa---UCGCGAGc -5' |
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| 8152 | 5' | -59.5 | NC_001978.2 | + | 40010 | 0.7 | 0.240844 |
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Target: 5'- cGACCGGCCcuaagucgUG-GUGGG-GGCGCgCGu -3' miRNA: 3'- aCUGGCCGG--------ACuCACCCaUCGCGaGC- -5' |
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| 8152 | 5' | -59.5 | NC_001978.2 | + | 39415 | 0.72 | 0.175362 |
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Target: 5'- cGACCGGCCgGAGcgugaucGGGUcGCGCUg- -3' miRNA: 3'- aCUGGCCGGaCUCa------CCCAuCGCGAgc -5' |
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| 8152 | 5' | -59.5 | NC_001978.2 | + | 40665 | 1.09 | 0.000269 |
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Target: 5'- gUGACCGGCCUGAGUGGGUAGCGCUCGa -3' miRNA: 3'- -ACUGGCCGGACUCACCCAUCGCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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