Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8154 | 5' | -55.2 | NC_001978.2 | + | 12675 | 0.66 | 0.673485 |
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Target: 5'- aUCUgUACGuUCGGGAaucguCGCUGGGUugGg -3' miRNA: 3'- -AGGgAUGUcAGCUCU-----GUGGCCCAugC- -5' |
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| 8154 | 5' | -55.2 | NC_001978.2 | + | 22139 | 0.67 | 0.617961 |
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Target: 5'- gCCCUAC-GUCGAGcACuuCCGGGa--- -3' miRNA: 3'- aGGGAUGuCAGCUC-UGu-GGCCCaugc -5' |
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| 8154 | 5' | -55.2 | NC_001978.2 | + | 29961 | 0.67 | 0.595769 |
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Target: 5'- cUCCCUugGGUUGucucGACuuCGGGUAa- -3' miRNA: 3'- -AGGGAugUCAGCu---CUGugGCCCAUgc -5' |
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| 8154 | 5' | -55.2 | NC_001978.2 | + | 14149 | 0.67 | 0.594662 |
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Target: 5'- aCCCgcCGGUCGAGugGucggcuaUCGGGcGCGg -3' miRNA: 3'- aGGGauGUCAGCUCugU-------GGCCCaUGC- -5' |
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| 8154 | 5' | -55.2 | NC_001978.2 | + | 9789 | 0.67 | 0.584716 |
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Target: 5'- aCCCgucgucgGCcGUUGAGACGCUGGGcaaggucgACGg -3' miRNA: 3'- aGGGa------UGuCAGCUCUGUGGCCCa-------UGC- -5' |
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| 8154 | 5' | -55.2 | NC_001978.2 | + | 29921 | 0.67 | 0.584716 |
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Target: 5'- aCCCggGCAGg-GAGugACCGGGgucacugaccgUGCGa -3' miRNA: 3'- aGGGa-UGUCagCUCugUGGCCC-----------AUGC- -5' |
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| 8154 | 5' | -55.2 | NC_001978.2 | + | 16030 | 0.7 | 0.437575 |
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Target: 5'- gUCCCcAgGGUCcgaaGGGugAcCCGGGUACGg -3' miRNA: 3'- -AGGGaUgUCAG----CUCugU-GGCCCAUGC- -5' |
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| 8154 | 5' | -55.2 | NC_001978.2 | + | 4058 | 0.71 | 0.399467 |
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Target: 5'- cCCCUGCccgacggauuGGgCGAGAagUACCGGGUGCc -3' miRNA: 3'- aGGGAUG----------UCaGCUCU--GUGGCCCAUGc -5' |
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| 8154 | 5' | -55.2 | NC_001978.2 | + | 41128 | 1.09 | 0.000891 |
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Target: 5'- gUCCCUACAGUCGAGACACCGGGUACGc -3' miRNA: 3'- -AGGGAUGUCAGCUCUGUGGCCCAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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