Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8155 | 3' | -58.4 | NC_001978.2 | + | 14470 | 0.66 | 0.477693 |
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Target: 5'- aAGCCa-ACGGACguaaaCCGUGCCCAG-CGc -3' miRNA: 3'- -UUGGcaUGUCUGg----GGCACGGGUCaGC- -5' |
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| 8155 | 3' | -58.4 | NC_001978.2 | + | 11608 | 0.66 | 0.474644 |
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Target: 5'- cACCGUGCGGGCagCGUcGCCCAcgauguaaggcgacGUCGc -3' miRNA: 3'- uUGGCAUGUCUGggGCA-CGGGU--------------CAGC- -5' |
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| 8155 | 3' | -58.4 | NC_001978.2 | + | 9798 | 0.66 | 0.467569 |
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Target: 5'- cGGCCGUugAGACgCUGgGCaaGGUCGa -3' miRNA: 3'- -UUGGCAugUCUGgGGCaCGggUCAGC- -5' |
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| 8155 | 3' | -58.4 | NC_001978.2 | + | 2943 | 0.66 | 0.467569 |
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Target: 5'- ---aGUGCAGACCaaaaacccaCCGUGCCCccuucgGGUCu -3' miRNA: 3'- uuggCAUGUCUGG---------GGCACGGG------UCAGc -5' |
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| 8155 | 3' | -58.4 | NC_001978.2 | + | 28236 | 0.66 | 0.437883 |
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Target: 5'- cGCCGUAUAGAUCaCGUcgaGCCCGG-CGu -3' miRNA: 3'- uUGGCAUGUCUGGgGCA---CGGGUCaGC- -5' |
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| 8155 | 3' | -58.4 | NC_001978.2 | + | 32333 | 0.66 | 0.437883 |
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Target: 5'- -uCCGUccGCuGGGCgUCGUGaCCCGGUCGa -3' miRNA: 3'- uuGGCA--UG-UCUGgGGCAC-GGGUCAGC- -5' |
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| 8155 | 3' | -58.4 | NC_001978.2 | + | 11510 | 0.67 | 0.376594 |
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Target: 5'- cGACCGcuUGC-GugCCCGUGCCaucacgaaggccguuCGGUCGg -3' miRNA: 3'- -UUGGC--AUGuCugGGGCACGG---------------GUCAGC- -5' |
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| 8155 | 3' | -58.4 | NC_001978.2 | + | 32102 | 0.73 | 0.172886 |
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Target: 5'- cGACCGUGCcgugauGACCCCG-GCUCAGgcgCGu -3' miRNA: 3'- -UUGGCAUGu-----CUGGGGCaCGGGUCa--GC- -5' |
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| 8155 | 3' | -58.4 | NC_001978.2 | + | 776 | 1.05 | 0.000634 |
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Target: 5'- uAACCGUACAGACCCCGUGCCCAGUCGc -3' miRNA: 3'- -UUGGCAUGUCUGGGGCACGGGUCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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