Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8157 | 3' | -55.2 | NC_001978.2 | + | 17111 | 0.7 | 0.433964 |
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Target: 5'- cGGCuaucACGAAGGCCGGU----CGGGCGg -3' miRNA: 3'- -CCG----UGCUUCCGGCCAuuggGCUUGCa -5' |
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| 8157 | 3' | -55.2 | NC_001978.2 | + | 11025 | 0.71 | 0.377837 |
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Target: 5'- gGGCGCGAAGucGCCuGGgcGCUgCGGACGUc -3' miRNA: 3'- -CCGUGCUUC--CGG-CCauUGG-GCUUGCA- -5' |
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| 8157 | 3' | -55.2 | NC_001978.2 | + | 4105 | 0.71 | 0.376944 |
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Target: 5'- gGGCgaagucgGCGAAGaGCCGG-AGCCUGAGCc- -3' miRNA: 3'- -CCG-------UGCUUC-CGGCCaUUGGGCUUGca -5' |
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| 8157 | 3' | -55.2 | NC_001978.2 | + | 16415 | 0.71 | 0.368969 |
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Target: 5'- gGGCuuCGAAgcguGGUCGGUcuACCCGGGCGg -3' miRNA: 3'- -CCGu-GCUU----CCGGCCAu-UGGGCUUGCa -5' |
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| 8157 | 3' | -55.2 | NC_001978.2 | + | 33493 | 0.75 | 0.222184 |
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Target: 5'- cGCGCGAAGGUgCGGUaugcgGACCCGGAgGa -3' miRNA: 3'- cCGUGCUUCCG-GCCA-----UUGGGCUUgCa -5' |
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| 8157 | 3' | -55.2 | NC_001978.2 | + | 5223 | 1.09 | 0.000773 |
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Target: 5'- cGGCACGAAGGCCGGUAACCCGAACGUu -3' miRNA: 3'- -CCGUGCUUCCGGCCAUUGGGCUUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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