Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
8157 | 5' | -55.3 | NC_001978.2 | + | 5306 | 0.66 | 0.603609 |
Target: 5'- -aAgGGUcCGGCUCAgaaCGUUCGGGUUa -3' miRNA: 3'- aaUgCCA-GUCGAGUa--GCGAGUCCGAc -5' |
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8157 | 5' | -55.3 | NC_001978.2 | + | 28912 | 0.68 | 0.429975 |
Target: 5'- -cACGGcUCAGCUCAUCaagggGCUUgAGGCg- -3' miRNA: 3'- aaUGCC-AGUCGAGUAG-----CGAG-UCCGac -5' |
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8157 | 5' | -55.3 | NC_001978.2 | + | 12131 | 0.68 | 0.428977 |
Target: 5'- gUUGCGGUCGGCUCcggcacgguccuuAUCGggggCAcGGCUGg -3' miRNA: 3'- -AAUGCCAGUCGAG-------------UAGCga--GU-CCGAC- -5' |
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8157 | 5' | -55.3 | NC_001978.2 | + | 25423 | 0.71 | 0.304668 |
Target: 5'- --cCGGUUcgGGCUCgacGUCGCcCAGGCUGa -3' miRNA: 3'- aauGCCAG--UCGAG---UAGCGaGUCCGAC- -5' |
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8157 | 5' | -55.3 | NC_001978.2 | + | 5264 | 1.05 | 0.000945 |
Target: 5'- uUUACGGUCAGCUCAUCGCUCAGGCUGu -3' miRNA: 3'- -AAUGCCAGUCGAGUAGCGAGUCCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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