Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8158 | 3' | -60.1 | NC_001978.2 | + | 25710 | 0.66 | 0.453846 |
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Target: 5'- aGGGUcCGGGCGACgcgcucacUGCCGuaucgGUCGg -3' miRNA: 3'- -CCCA-GCCCGCUG--------GCGGCccuaaCAGC- -5' |
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| 8158 | 3' | -60.1 | NC_001978.2 | + | 9979 | 0.67 | 0.38955 |
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Target: 5'- cGGUCGacuauGGCGACgGUCGGcGGcuucgUGUCGg -3' miRNA: 3'- cCCAGC-----CCGCUGgCGGCC-CUa----ACAGC- -5' |
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| 8158 | 3' | -60.1 | NC_001978.2 | + | 22333 | 0.67 | 0.355587 |
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Target: 5'- cGGcGUCGGGUuGCCGUCGGc---GUCGa -3' miRNA: 3'- -CC-CAGCCCGcUGGCGGCCcuaaCAGC- -5' |
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| 8158 | 3' | -60.1 | NC_001978.2 | + | 24778 | 0.68 | 0.347429 |
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Target: 5'- -cGUCGGGCGACgGCauaCGGG---GUCGa -3' miRNA: 3'- ccCAGCCCGCUGgCG---GCCCuaaCAGC- -5' |
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| 8158 | 3' | -60.1 | NC_001978.2 | + | 1959 | 0.69 | 0.30132 |
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Target: 5'- gGGGUCGGGUccGGCCGUCGaGAUgaUCa -3' miRNA: 3'- -CCCAGCCCG--CUGGCGGCcCUAacAGc -5' |
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| 8158 | 3' | -60.1 | NC_001978.2 | + | 20642 | 0.69 | 0.29411 |
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Target: 5'- aGGUCGGGCaGACCggucGCCGGGu---UCa -3' miRNA: 3'- cCCAGCCCG-CUGG----CGGCCCuaacAGc -5' |
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| 8158 | 3' | -60.1 | NC_001978.2 | + | 36055 | 0.7 | 0.260078 |
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Target: 5'- cGGGUC-GGCGGCagucuCCGGGG-UGUCGc -3' miRNA: 3'- -CCCAGcCCGCUGgc---GGCCCUaACAGC- -5' |
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| 8158 | 3' | -60.1 | NC_001978.2 | + | 8184 | 0.75 | 0.118391 |
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Target: 5'- cGGGUCGcGGUGACCGuCCGGGcUU-UCGu -3' miRNA: 3'- -CCCAGC-CCGCUGGC-GGCCCuAAcAGC- -5' |
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| 8158 | 3' | -60.1 | NC_001978.2 | + | 5372 | 1.11 | 0.000211 |
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Target: 5'- cGGGUCGGGCGACCGCCGGGAUUGUCGg -3' miRNA: 3'- -CCCAGCCCGCUGGCGGCCCUAACAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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