Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8162 | 3' | -55.8 | NC_001978.2 | + | 15738 | 0.66 | 0.584491 |
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Target: 5'- gGCgaagucgGGCGGCGUGUggucgaaggugucggACGGCGUUCg-- -3' miRNA: 3'- gUGa------CUGCCGCGCA---------------UGUCGCGAGauu -5' |
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| 8162 | 3' | -55.8 | NC_001978.2 | + | 10736 | 0.67 | 0.512461 |
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Target: 5'- cCGCUGugGG-GCGUGCcgcgaagGGCGCUa--- -3' miRNA: 3'- -GUGACugCCgCGCAUG-------UCGCGAgauu -5' |
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| 8162 | 3' | -55.8 | NC_001978.2 | + | 26059 | 0.68 | 0.460662 |
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Target: 5'- gACuUGACGGCGuCGUGCAGUcgGCUg--- -3' miRNA: 3'- gUG-ACUGCCGC-GCAUGUCG--CGAgauu -5' |
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| 8162 | 3' | -55.8 | NC_001978.2 | + | 26728 | 0.68 | 0.45043 |
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Target: 5'- aCGCUGACGGaCGUGUucgGGCGCaUCa-- -3' miRNA: 3'- -GUGACUGCC-GCGCAug-UCGCG-AGauu -5' |
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| 8162 | 3' | -55.8 | NC_001978.2 | + | 29898 | 0.75 | 0.15957 |
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Target: 5'- uCACUGACcGUGCGacgGCAGCGCUUUGc -3' miRNA: 3'- -GUGACUGcCGCGCa--UGUCGCGAGAUu -5' |
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| 8162 | 3' | -55.8 | NC_001978.2 | + | 8903 | 0.78 | 0.103575 |
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Target: 5'- --aUGACGG-GCGUGCGGCGCUCa-- -3' miRNA: 3'- gugACUGCCgCGCAUGUCGCGAGauu -5' |
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| 8162 | 3' | -55.8 | NC_001978.2 | + | 6886 | 1.05 | 0.000907 |
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Target: 5'- cCACUGACGGCGCGUACAGCGCUCUAAc -3' miRNA: 3'- -GUGACUGCCGCGCAUGUCGCGAGAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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