Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8170 | 5' | -49.1 | NC_001978.2 | + | 965 | 0.66 | 0.929435 |
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Target: 5'- -cGCuuCAUG-CGGUCGcGCUUcucgGCGg -3' miRNA: 3'- caCGuuGUACaGCCAGCuCGAAa---UGC- -5' |
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| 8170 | 5' | -49.1 | NC_001978.2 | + | 7549 | 0.66 | 0.923295 |
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Target: 5'- cGUGCgGGCAgcgUGGUCGAGUUcgACGc -3' miRNA: 3'- -CACG-UUGUacaGCCAGCUCGAaaUGC- -5' |
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| 8170 | 5' | -49.1 | NC_001978.2 | + | 17859 | 0.66 | 0.91684 |
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Target: 5'- -cGCGGCAUaGUUGGUCGuGUcg-GCGu -3' miRNA: 3'- caCGUUGUA-CAGCCAGCuCGaaaUGC- -5' |
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| 8170 | 5' | -49.1 | NC_001978.2 | + | 15724 | 0.67 | 0.902991 |
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Target: 5'- -gGCGuACGUGUCGccGUCGAGCcccaGCGa -3' miRNA: 3'- caCGU-UGUACAGC--CAGCUCGaaa-UGC- -5' |
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| 8170 | 5' | -49.1 | NC_001978.2 | + | 35824 | 0.67 | 0.895601 |
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Target: 5'- -gGCGGgAg--UGGUCGAGCUggGCGa -3' miRNA: 3'- caCGUUgUacaGCCAGCUCGAaaUGC- -5' |
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| 8170 | 5' | -49.1 | NC_001978.2 | + | 36121 | 0.68 | 0.845124 |
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Target: 5'- cGUGUAGCAcGUCGG-CGAGUaggGCa -3' miRNA: 3'- -CACGUUGUaCAGCCaGCUCGaaaUGc -5' |
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| 8170 | 5' | -49.1 | NC_001978.2 | + | 27137 | 0.69 | 0.816318 |
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Target: 5'- gGUGCGcgGUG-CGGUCGAGUgguaUGCGa -3' miRNA: 3'- -CACGUugUACaGCCAGCUCGaa--AUGC- -5' |
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| 8170 | 5' | -49.1 | NC_001978.2 | + | 39360 | 0.69 | 0.806258 |
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Target: 5'- -cGCu-CcgGcCGGUCGAGCUUgauUGCGg -3' miRNA: 3'- caCGuuGuaCaGCCAGCUCGAA---AUGC- -5' |
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| 8170 | 5' | -49.1 | NC_001978.2 | + | 35726 | 0.74 | 0.521919 |
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Target: 5'- aGUGcCGACcgGUCuucggGGUCGAGCUggGCGc -3' miRNA: 3'- -CAC-GUUGuaCAG-----CCAGCUCGAaaUGC- -5' |
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| 8170 | 5' | -49.1 | NC_001978.2 | + | 11501 | 1.09 | 0.002919 |
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Target: 5'- cGUGCAACAUGUCGGUCGAGCUUUACGc -3' miRNA: 3'- -CACGUUGUACAGCCAGCUCGAAAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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