Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8176 | 3' | -55.6 | NC_001978.2 | + | 26312 | 0.67 | 0.56863 |
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Target: 5'- cGGCGCUUgugcucGCCGGUUGCCugcCGgagUCgCg -3' miRNA: 3'- -UCGCGGA------CGGCUAACGGuu-GCa--AGgG- -5' |
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| 8176 | 3' | -55.6 | NC_001978.2 | + | 33534 | 0.67 | 0.546596 |
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Target: 5'- aAGCGCgUUGCCGc-UGCCAuuGCGggugucgCCCu -3' miRNA: 3'- -UCGCG-GACGGCuaACGGU--UGCaa-----GGG- -5' |
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| 8176 | 3' | -55.6 | NC_001978.2 | + | 22994 | 0.67 | 0.535681 |
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Target: 5'- cGCGUUguaccGCCcgGGUUGCCGACG-UCCUu -3' miRNA: 3'- uCGCGGa----CGG--CUAACGGUUGCaAGGG- -5' |
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| 8176 | 3' | -55.6 | NC_001978.2 | + | 16090 | 0.68 | 0.482397 |
|
Target: 5'- uAGgGCCggauaucacccuUGCCGc-UGCCGACGUcggggcgauUCCCg -3' miRNA: 3'- -UCgCGG------------ACGGCuaACGGUUGCA---------AGGG- -5' |
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| 8176 | 3' | -55.6 | NC_001978.2 | + | 15945 | 0.68 | 0.472043 |
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Target: 5'- gGGCGCgacgggcGCCGAc--UCGACGUUCCCg -3' miRNA: 3'- -UCGCGga-----CGGCUaacGGUUGCAAGGG- -5' |
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| 8176 | 3' | -55.6 | NC_001978.2 | + | 35212 | 0.69 | 0.431807 |
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Target: 5'- gGGCGCCggaagGacaCGGgcGCCAugGUUgCCg -3' miRNA: 3'- -UCGCGGa----Cg--GCUaaCGGUugCAAgGG- -5' |
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| 8176 | 3' | -55.6 | NC_001978.2 | + | 16350 | 1.12 | 0.000349 |
|
Target: 5'- aAGCGCCUGCCGAUUGCCAACGUUCCCa -3' miRNA: 3'- -UCGCGGACGGCUAACGGUUGCAAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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