Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8176 | 5' | -50.9 | NC_001978.2 | + | 8968 | 0.66 | 0.884269 |
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Target: 5'- uCGgcGAACAGgcgagGGGCUugcccuugCUGCGGGUGg -3' miRNA: 3'- -GCuuCUUGUCaa---CCUGA--------GGCGUUCGC- -5' |
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| 8176 | 5' | -50.9 | NC_001978.2 | + | 12593 | 0.66 | 0.841636 |
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Target: 5'- aCGAAGGACccgGGUccgUGGAagaagugcaCUCCGCAgaacguacaGGCGa -3' miRNA: 3'- -GCUUCUUG---UCA---ACCU---------GAGGCGU---------UCGC- -5' |
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| 8176 | 5' | -50.9 | NC_001978.2 | + | 6972 | 0.67 | 0.832343 |
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Target: 5'- aGggGuuuuGCA--UGGACUCgaCGCAAGCa -3' miRNA: 3'- gCuuCu---UGUcaACCUGAG--GCGUUCGc -5' |
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| 8176 | 5' | -50.9 | NC_001978.2 | + | 18914 | 0.68 | 0.772108 |
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Target: 5'- aCGAcGGGCAGgcucaGGcGCUCCGCA-GCGu -3' miRNA: 3'- -GCUuCUUGUCaa---CC-UGAGGCGUuCGC- -5' |
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| 8176 | 5' | -50.9 | NC_001978.2 | + | 16308 | 0.72 | 0.512139 |
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Target: 5'- aCGAAGAACgcugcggacggAGUUGccccgcucGACUCCGCgAAGCGc -3' miRNA: 3'- -GCUUCUUG-----------UCAAC--------CUGAGGCG-UUCGC- -5' |
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| 8176 | 5' | -50.9 | NC_001978.2 | + | 41367 | 0.74 | 0.417763 |
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Target: 5'- aCGAAG-ACGGcaaUGGACUUCGgCAAGCGu -3' miRNA: 3'- -GCUUCuUGUCa--ACCUGAGGC-GUUCGC- -5' |
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| 8176 | 5' | -50.9 | NC_001978.2 | + | 7055 | 0.76 | 0.326314 |
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Target: 5'- aCGAGGGGCAGgcGGACUCuUGgAAGCa -3' miRNA: 3'- -GCUUCUUGUCaaCCUGAG-GCgUUCGc -5' |
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| 8176 | 5' | -50.9 | NC_001978.2 | + | 25070 | 0.84 | 0.112753 |
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Target: 5'- aGggGAGuCAGUaUGGACUUCGCAAGCa -3' miRNA: 3'- gCuuCUU-GUCA-ACCUGAGGCGUUCGc -5' |
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| 8176 | 5' | -50.9 | NC_001978.2 | + | 16386 | 1.09 | 0.002008 |
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Target: 5'- cCGAAGAACAGUUGGACUCCGCAAGCGc -3' miRNA: 3'- -GCUUCUUGUCAACCUGAGGCGUUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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