Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8177 | 5' | -54.2 | NC_001978.2 | + | 32464 | 0.71 | 0.387232 |
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Target: 5'- -uGGCuUCACGGuGCCGAcuCGACCGa- -3' miRNA: 3'- agUUGuAGUGCCuCGGCU--GCUGGCau -5' |
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| 8177 | 5' | -54.2 | NC_001978.2 | + | 32108 | 0.71 | 0.378081 |
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Target: 5'- aCGACuUCACGaAGCCGACGACgCGc- -3' miRNA: 3'- aGUUGuAGUGCcUCGGCUGCUG-GCau -5' |
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| 8177 | 5' | -54.2 | NC_001978.2 | + | 36724 | 0.71 | 0.374461 |
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Target: 5'- ---cCGUCACGGAGCCGGagacggcgccccaGGCCGUu -3' miRNA: 3'- aguuGUAGUGCCUCGGCUg------------CUGGCAu -5' |
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| 8177 | 5' | -54.2 | NC_001978.2 | + | 17567 | 0.72 | 0.351512 |
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Target: 5'- gCAACGUCugGGuAGCCGua-GCCGUAc -3' miRNA: 3'- aGUUGUAGugCC-UCGGCugcUGGCAU- -5' |
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| 8177 | 5' | -54.2 | NC_001978.2 | + | 13642 | 0.72 | 0.334549 |
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Target: 5'- gUCAGCGcgaACGGAGUCGACG-CCGa- -3' miRNA: 3'- -AGUUGUag-UGCCUCGGCUGCuGGCau -5' |
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| 8177 | 5' | -54.2 | NC_001978.2 | + | 36761 | 0.74 | 0.258825 |
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Target: 5'- -gGACGUCcuucCGGAGCCGACG-CCGg- -3' miRNA: 3'- agUUGUAGu---GCCUCGGCUGCuGGCau -5' |
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| 8177 | 5' | -54.2 | NC_001978.2 | + | 30238 | 0.75 | 0.245471 |
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Target: 5'- aCGGCAaCGcCGGGGCCGACGaaaacGCCGUGa -3' miRNA: 3'- aGUUGUaGU-GCCUCGGCUGC-----UGGCAU- -5' |
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| 8177 | 5' | -54.2 | NC_001978.2 | + | 39132 | 0.75 | 0.23207 |
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Target: 5'- gCAGCAUCACGGGGCUuuguaagcgcaugGACGcugacGCCGUGc -3' miRNA: 3'- aGUUGUAGUGCCUCGG-------------CUGC-----UGGCAU- -5' |
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| 8177 | 5' | -54.2 | NC_001978.2 | + | 16503 | 1.08 | 0.001149 |
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Target: 5'- uUCAACAUCACGGAGCCGACGACCGUAa -3' miRNA: 3'- -AGUUGUAGUGCCUCGGCUGCUGGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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