Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8178 | 5' | -54.6 | NC_001978.2 | + | 20929 | 0.66 | 0.667077 |
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Target: 5'- cCUGCCCg--GCGUacaGCCGguaaaugGCUUCGg -3' miRNA: 3'- -GACGGGuaaCGCAgc-UGGCa------CGAAGU- -5' |
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| 8178 | 5' | -54.6 | NC_001978.2 | + | 40878 | 0.67 | 0.621776 |
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Target: 5'- -cGCUCAgcGUGUCGcCCG-GCUUCu -3' miRNA: 3'- gaCGGGUaaCGCAGCuGGCaCGAAGu -5' |
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| 8178 | 5' | -54.6 | NC_001978.2 | + | 28017 | 0.68 | 0.521324 |
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Target: 5'- -cGCCUuaaucuucGUGUCGACCGUGCg--- -3' miRNA: 3'- gaCGGGuaa-----CGCAGCUGGCACGaagu -5' |
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| 8178 | 5' | -54.6 | NC_001978.2 | + | 39940 | 0.68 | 0.521324 |
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Target: 5'- gCUGCCUGUUGCuucCGGaaGUGCUUCc -3' miRNA: 3'- -GACGGGUAACGca-GCUggCACGAAGu -5' |
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| 8178 | 5' | -54.6 | NC_001978.2 | + | 9291 | 0.69 | 0.489141 |
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Target: 5'- aUGUucgCCAUUGuCGUCGGCCGUcGCUcCGg -3' miRNA: 3'- gACG---GGUAAC-GCAGCUGGCA-CGAaGU- -5' |
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| 8178 | 5' | -54.6 | NC_001978.2 | + | 1905 | 0.71 | 0.389514 |
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Target: 5'- aCUGCCCAgcgucagcGCGUCGaauaggucGCUGUGCUUg- -3' miRNA: 3'- -GACGGGUaa------CGCAGC--------UGGCACGAAgu -5' |
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| 8178 | 5' | -54.6 | NC_001978.2 | + | 32433 | 0.72 | 0.336466 |
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Target: 5'- -cGCCCuuccaUGCGUCGAgCCGUucggcggacGCUUCAg -3' miRNA: 3'- gaCGGGua---ACGCAGCU-GGCA---------CGAAGU- -5' |
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| 8178 | 5' | -54.6 | NC_001978.2 | + | 16721 | 1.09 | 0.000786 |
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Target: 5'- gCUGCCCAUUGCGUCGACCGUGCUUCAg -3' miRNA: 3'- -GACGGGUAACGCAGCUGGCACGAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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