Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8182 | 5' | -57.3 | NC_001978.2 | + | 15781 | 0.69 | 0.33307 |
Target: 5'- cGUGGCgCaGCGUGGuacACGAAcaacACGGGCAc -3' miRNA: 3'- -CACCG-GaUGCACC---UGCUUc---UGCCCGU- -5' |
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8182 | 5' | -57.3 | NC_001978.2 | + | 29452 | 0.72 | 0.233122 |
Target: 5'- -aGGUCaGCGUcGGCGAcGACGGGCAc -3' miRNA: 3'- caCCGGaUGCAcCUGCUuCUGCCCGU- -5' |
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8182 | 5' | -57.3 | NC_001978.2 | + | 21614 | 0.77 | 0.107713 |
Target: 5'- -gGGCUgUugG-GGGCGGAGACGGGCGc -3' miRNA: 3'- caCCGG-AugCaCCUGCUUCUGCCCGU- -5' |
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8182 | 5' | -57.3 | NC_001978.2 | + | 15725 | 0.79 | 0.07408 |
Target: 5'- -cGGUcaCUAUGUGGGCGAAGuCGGGCGg -3' miRNA: 3'- caCCG--GAUGCACCUGCUUCuGCCCGU- -5' |
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8182 | 5' | -57.3 | NC_001978.2 | + | 19566 | 1.08 | 0.000497 |
Target: 5'- gGUGGCCUACGUGGACGAAGACGGGCAg -3' miRNA: 3'- -CACCGGAUGCACCUGCUUCUGCCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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