Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
8184 | 5' | -51.6 | NC_001978.2 | + | 35816 | 0.66 | 0.831318 |
Target: 5'- uGGUCGAGcugggcGACGGCGUugcCAAGggCUUCg -3' miRNA: 3'- -CUAGUUC------CUGCCGCA---GUUCgaGAAGa -5' |
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8184 | 5' | -51.6 | NC_001978.2 | + | 20417 | 0.66 | 0.811896 |
Target: 5'- --cCGGGGugacCGGCGUC-AGCUCcgUCUc -3' miRNA: 3'- cuaGUUCCu---GCCGCAGuUCGAGa-AGA- -5' |
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8184 | 5' | -51.6 | NC_001978.2 | + | 35984 | 0.66 | 0.811896 |
Target: 5'- cGAUCAAGGGCGucucaGCGUC-GGCUUc--- -3' miRNA: 3'- -CUAGUUCCUGC-----CGCAGuUCGAGaaga -5' |
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8184 | 5' | -51.6 | NC_001978.2 | + | 27014 | 0.66 | 0.801863 |
Target: 5'- gGAUgAGGGGCuuuGGCGUCGcgugaAGCUCaUUCc -3' miRNA: 3'- -CUAgUUCCUG---CCGCAGU-----UCGAG-AAGa -5' |
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8184 | 5' | -51.6 | NC_001978.2 | + | 11997 | 0.67 | 0.781216 |
Target: 5'- -uUC-AGGACGGCGUCGgcuauGGCgcgUCUc -3' miRNA: 3'- cuAGuUCCUGCCGCAGU-----UCGagaAGA- -5' |
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8184 | 5' | -51.6 | NC_001978.2 | + | 9699 | 0.68 | 0.681315 |
Target: 5'- aGAUCAAGGAccCGGCGgagcguaucgCGAccgcugugacGCUCUUCg -3' miRNA: 3'- -CUAGUUCCU--GCCGCa---------GUU----------CGAGAAGa -5' |
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8184 | 5' | -51.6 | NC_001978.2 | + | 40585 | 0.69 | 0.623353 |
Target: 5'- --cUAAGGaACGGCGUCGAGCgCUa-- -3' miRNA: 3'- cuaGUUCC-UGCCGCAGUUCGaGAaga -5' |
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8184 | 5' | -51.6 | NC_001978.2 | + | 27138 | 0.73 | 0.424732 |
Target: 5'- cGGUCAAGGuCGuaGUCGcGCUCUUCc -3' miRNA: 3'- -CUAGUUCCuGCcgCAGUuCGAGAAGa -5' |
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8184 | 5' | -51.6 | NC_001978.2 | + | 20464 | 1.07 | 0.001994 |
Target: 5'- uGAUCAAGGACGGCGUCAAGCUCUUCUc -3' miRNA: 3'- -CUAGUUCCUGCCGCAGUUCGAGAAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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