Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8186 | 3' | -54.1 | NC_001978.2 | + | 17436 | 0.73 | 0.345869 |
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Target: 5'- aCGAACG-GcAGCGcuGAGGGCGAcGGCGu -3' miRNA: 3'- -GCUUGCuCuUCGC--CUUCCGCUuCCGC- -5' |
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| 8186 | 3' | -54.1 | NC_001978.2 | + | 4655 | 0.73 | 0.321072 |
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Target: 5'- cCGu-CGAGcacGCGGAAGGUGAAGGaCGa -3' miRNA: 3'- -GCuuGCUCuu-CGCCUUCCGCUUCC-GC- -5' |
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| 8186 | 3' | -54.1 | NC_001978.2 | + | 24454 | 0.73 | 0.321072 |
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Target: 5'- cCGGGCGccacuGAGCGGAAGGacuGggGGCGc -3' miRNA: 3'- -GCUUGCuc---UUCGCCUUCCg--CuuCCGC- -5' |
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| 8186 | 3' | -54.1 | NC_001978.2 | + | 37841 | 0.73 | 0.313107 |
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Target: 5'- cCGGACGuGggGCGaagugccccuuGucGGCGggGGCGc -3' miRNA: 3'- -GCUUGCuCuuCGC-----------CuuCCGCuuCCGC- -5' |
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| 8186 | 3' | -54.1 | NC_001978.2 | + | 8630 | 0.73 | 0.313107 |
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Target: 5'- uGAACcuGAAGgGGAAGGCGAAGcCGg -3' miRNA: 3'- gCUUGcuCUUCgCCUUCCGCUUCcGC- -5' |
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| 8186 | 3' | -54.1 | NC_001978.2 | + | 21194 | 1.1 | 0.000854 |
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Target: 5'- gCGAACGAGAAGCGGAAGGCGAAGGCGg -3' miRNA: 3'- -GCUUGCUCUUCGCCUUCCGCUUCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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