Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8189 | 3' | -55.9 | NC_001978.2 | + | 16407 | 0.66 | 0.604532 |
Target: 5'- cAAGcgCUGgGCUucgaagcguggucgGUCUACCcGGGCGGc -3' miRNA: 3'- -UUCuaGGCgUGA--------------UAGAUGGcCCCGCC- -5' |
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8189 | 3' | -55.9 | NC_001978.2 | + | 2893 | 0.66 | 0.574468 |
Target: 5'- -uGGUCUGCACUcuUCggGCgGGGGCa- -3' miRNA: 3'- uuCUAGGCGUGAu-AGa-UGgCCCCGcc -5' |
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8189 | 3' | -55.9 | NC_001978.2 | + | 33945 | 0.66 | 0.574468 |
Target: 5'- aGAGAUCacgGCGCU-UCUgaACCGGGGUc- -3' miRNA: 3'- -UUCUAGg--CGUGAuAGA--UGGCCCCGcc -5' |
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8189 | 3' | -55.9 | NC_001978.2 | + | 24825 | 0.67 | 0.519854 |
Target: 5'- aGAGAUCaugcgGCGCUGgggCUACCGGuGCGc -3' miRNA: 3'- -UUCUAGg----CGUGAUa--GAUGGCCcCGCc -5' |
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8189 | 3' | -55.9 | NC_001978.2 | + | 32142 | 0.68 | 0.484942 |
Target: 5'- ----gCCGCAUgg-CUgaacgaggaagccgACCGGGGCGGa -3' miRNA: 3'- uucuaGGCGUGauaGA--------------UGGCCCCGCC- -5' |
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8189 | 3' | -55.9 | NC_001978.2 | + | 1154 | 0.68 | 0.447185 |
Target: 5'- --cGUCCGCGUUGUgCU-CCGGGGCGa -3' miRNA: 3'- uucUAGGCGUGAUA-GAuGGCCCCGCc -5' |
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8189 | 3' | -55.9 | NC_001978.2 | + | 28974 | 0.75 | 0.18455 |
Target: 5'- cGGAUUCgGCAagGUCUACgGGGGCGGa -3' miRNA: 3'- uUCUAGG-CGUgaUAGAUGgCCCCGCC- -5' |
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8189 | 3' | -55.9 | NC_001978.2 | + | 21804 | 1.07 | 0.000772 |
Target: 5'- gAAGAUCCGCACUAUCUACCGGGGCGGc -3' miRNA: 3'- -UUCUAGGCGUGAUAGAUGGCCCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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