Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8190 | 3' | -54 | NC_001978.2 | + | 11534 | 0.66 | 0.721003 |
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Target: 5'- cGCCUuacAUCGUGGGCGACgcugcccgcacGGu-GACGg -3' miRNA: 3'- -UGGAc--UAGUACCCGCUGa----------CCuuCUGC- -5' |
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| 8190 | 3' | -54 | NC_001978.2 | + | 7249 | 0.66 | 0.709972 |
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Target: 5'- -aCUGA-CAcGGGCGGC-GuGAAGACGa -3' miRNA: 3'- ugGACUaGUaCCCGCUGaC-CUUCUGC- -5' |
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| 8190 | 3' | -54 | NC_001978.2 | + | 30019 | 0.66 | 0.698861 |
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Target: 5'- gGCaaGGUCGUcGGCgGACUGGgcGGCGc -3' miRNA: 3'- -UGgaCUAGUAcCCG-CUGACCuuCUGC- -5' |
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| 8190 | 3' | -54 | NC_001978.2 | + | 16747 | 0.66 | 0.69216 |
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Target: 5'- aGCCUGggCGcuacUGGGUGACguggcuucaggugaaUGGggGcACGg -3' miRNA: 3'- -UGGACuaGU----ACCCGCUG---------------ACCuuC-UGC- -5' |
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| 8190 | 3' | -54 | NC_001978.2 | + | 995 | 0.67 | 0.608486 |
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Target: 5'- gUCUGG-CggGGGCGACgacccGGAAGGCu -3' miRNA: 3'- uGGACUaGuaCCCGCUGa----CCUUCUGc -5' |
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| 8190 | 3' | -54 | NC_001978.2 | + | 103 | 0.69 | 0.519379 |
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Target: 5'- gGCCUGGg---GGGCGcCUGGGuccGGCGg -3' miRNA: 3'- -UGGACUaguaCCCGCuGACCUu--CUGC- -5' |
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| 8190 | 3' | -54 | NC_001978.2 | + | 22311 | 1.08 | 0.001059 |
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Target: 5'- cACCUGAUCAUGGGCGACUGGAAGACGu -3' miRNA: 3'- -UGGACUAGUACCCGCUGACCUUCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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