Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8192 | 5' | -56.8 | NC_001978.2 | + | 20393 | 0.67 | 0.51462 |
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Target: 5'- gGCCgaaGUcgagucGCGcCGGUCGG-CcGACGUCg -3' miRNA: 3'- aCGGa--CA------UGC-GCCAGCCaGaCUGCAG- -5' |
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| 8192 | 5' | -56.8 | NC_001978.2 | + | 6921 | 0.67 | 0.493633 |
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Target: 5'- gUGCggGUGCGCGGUCGcGUCuaucagUGACa-- -3' miRNA: 3'- -ACGgaCAUGCGCCAGC-CAG------ACUGcag -5' |
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| 8192 | 5' | -56.8 | NC_001978.2 | + | 30941 | 0.67 | 0.483288 |
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Target: 5'- gGCaUGUGCGCccaGGUCGGcgUGACGUa -3' miRNA: 3'- aCGgACAUGCG---CCAGCCagACUGCAg -5' |
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| 8192 | 5' | -56.8 | NC_001978.2 | + | 16409 | 0.67 | 0.462917 |
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Target: 5'- aGCgCUGggcuucgaaGCGUGGUCGGUCUacccgggcGGCGUg -3' miRNA: 3'- aCG-GACa--------UGCGCCAGCCAGA--------CUGCAg -5' |
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| 8192 | 5' | -56.8 | NC_001978.2 | + | 27128 | 0.73 | 0.205196 |
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Target: 5'- gGCCUgggcgGUGCGCGGUgCGGUCgaGugGUa -3' miRNA: 3'- aCGGA-----CAUGCGCCA-GCCAGa-CugCAg -5' |
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| 8192 | 5' | -56.8 | NC_001978.2 | + | 22855 | 1.1 | 0.000432 |
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Target: 5'- cUGCCUGUACGCGGUCGGUCUGACGUCg -3' miRNA: 3'- -ACGGACAUGCGCCAGCCAGACUGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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