Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8194 | 3' | -58.5 | NC_001978.2 | + | 10200 | 0.67 | 0.379237 |
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Target: 5'- cGGGUGGAuagcgucgggcguucAGGCcggacucaacGCGGCGAAGGuGg -3' miRNA: 3'- aCUCGCCU---------------UCCGu---------CGCCGCUUCCuCa -5' |
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| 8194 | 3' | -58.5 | NC_001978.2 | + | 2585 | 0.68 | 0.30926 |
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Target: 5'- cUGAGUGGcAGGCAccuGCGcGCGAGaGAGUu -3' miRNA: 3'- -ACUCGCCuUCCGU---CGC-CGCUUcCUCA- -5' |
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| 8194 | 3' | -58.5 | NC_001978.2 | + | 21201 | 0.69 | 0.301616 |
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Target: 5'- aGaAGCGGAAGGCGaaGGCGGAGcuGUa -3' miRNA: 3'- aC-UCGCCUUCCGUcgCCGCUUCcuCA- -5' |
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| 8194 | 3' | -58.5 | NC_001978.2 | + | 40266 | 0.7 | 0.258143 |
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Target: 5'- -cAGCGGGcuuccugGGGCAGCGG-GAAGGGu- -3' miRNA: 3'- acUCGCCU-------UCCGUCGCCgCUUCCUca -5' |
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| 8194 | 3' | -58.5 | NC_001978.2 | + | 4192 | 0.7 | 0.239337 |
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Target: 5'- gGAGcCGGAAGGCaAGcCGGacgaCGAAGGGGc -3' miRNA: 3'- aCUC-GCCUUCCG-UC-GCC----GCUUCCUCa -5' |
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| 8194 | 3' | -58.5 | NC_001978.2 | + | 23499 | 1.08 | 0.000357 |
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Target: 5'- aUGAGCGGAAGGCAGCGGCGAAGGAGUa -3' miRNA: 3'- -ACUCGCCUUCCGUCGCCGCUUCCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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