Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8204 | 5' | -56.6 | NC_001978.2 | + | 25467 | 0.66 | 0.604384 |
Target: 5'- --cCGGUCGGcGGCGUAgUCggCGGc- -3' miRNA: 3'- ccaGCCAGCC-CCGCGUgAGuaGCUuc -5' |
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8204 | 5' | -56.6 | NC_001978.2 | + | 37814 | 0.66 | 0.601094 |
Target: 5'- gGGUCGccgucGUCGGGcagcGCGCaauagcccuucaugGCUCcGUCGAAGa -3' miRNA: 3'- -CCAGC-----CAGCCC----CGCG--------------UGAG-UAGCUUC- -5' |
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8204 | 5' | -56.6 | NC_001978.2 | + | 21583 | 0.66 | 0.590147 |
Target: 5'- cGUCGcGUacagccucagcgcaCGGGGUGCACgUCggCGGAGu -3' miRNA: 3'- cCAGC-CA--------------GCCCCGCGUG-AGuaGCUUC- -5' |
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8204 | 5' | -56.6 | NC_001978.2 | + | 40022 | 0.66 | 0.571624 |
Target: 5'- aGUCguGGUgGGGGCGCGCgUCAguagaCGAc- -3' miRNA: 3'- cCAG--CCAgCCCCGCGUG-AGUa----GCUuc -5' |
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8204 | 5' | -56.6 | NC_001978.2 | + | 40060 | 0.66 | 0.571624 |
Target: 5'- gGGcCGGUCGGGucaGCGUcgGCgUCuUCGggGa -3' miRNA: 3'- -CCaGCCAGCCC---CGCG--UG-AGuAGCuuC- -5' |
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8204 | 5' | -56.6 | NC_001978.2 | + | 40218 | 0.68 | 0.456728 |
Target: 5'- cGUCGGUCGGcGGCuucgccCACgUgAUCGAAGc -3' miRNA: 3'- cCAGCCAGCC-CCGc-----GUG-AgUAGCUUC- -5' |
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8204 | 5' | -56.6 | NC_001978.2 | + | 22585 | 0.7 | 0.363939 |
Target: 5'- cGGUCGcacggaaggcGUCGGGGCGCauggugaccggcACUCAgCGAc- -3' miRNA: 3'- -CCAGC----------CAGCCCCGCG------------UGAGUaGCUuc -5' |
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8204 | 5' | -56.6 | NC_001978.2 | + | 34249 | 0.71 | 0.338763 |
Target: 5'- aGUCGGagUUGGGcGCGCACUCGaCGGc- -3' miRNA: 3'- cCAGCC--AGCCC-CGCGUGAGUaGCUuc -5' |
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8204 | 5' | -56.6 | NC_001978.2 | + | 18543 | 0.72 | 0.257481 |
Target: 5'- -aUCGGUCuuaucGGGCGCGCUCAgUCGGGc -3' miRNA: 3'- ccAGCCAGc----CCCGCGUGAGU-AGCUUc -5' |
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8204 | 5' | -56.6 | NC_001978.2 | + | 30514 | 1.1 | 0.000526 |
Target: 5'- aGGUCGGUCGGGGCGCACUCAUCGAAGc -3' miRNA: 3'- -CCAGCCAGCCCCGCGUGAGUAGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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