Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8206 | 5' | -55.2 | NC_001978.2 | + | 23920 | 0.73 | 0.258825 |
Target: 5'- cACGGCGUCGuCAGAAGCgguGCGCaUGGu- -3' miRNA: 3'- cUGCCGCAGU-GUCUUCG---CGCG-ACUuc -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 6068 | 0.78 | 0.126025 |
Target: 5'- --gGGCGUCGacaAGAagaccgucGGCGCGCUGAAGg -3' miRNA: 3'- cugCCGCAGUg--UCU--------UCGCGCGACUUC- -5' |
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8206 | 5' | -55.2 | NC_001978.2 | + | 32333 | 1.08 | 0.000795 |
Target: 5'- aGACGGCGUCACAGAAGCGCGCUGAAGc -3' miRNA: 3'- -CUGCCGCAGUGUCUUCGCGCGACUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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