Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8208 | 3' | -56.7 | NC_001978.2 | + | 33003 | 1.1 | 0.000418 |
Target: 5'- gGGUGAAGCUUCUUCGAGCCCGGGGCUa -3' miRNA: 3'- -CCACUUCGAAGAAGCUCGGGCCCCGA- -5' |
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8208 | 3' | -56.7 | NC_001978.2 | + | 6005 | 0.71 | 0.272555 |
Target: 5'- --cGAcGGuCUUCUugUCGAcGCCCGGGGCg -3' miRNA: 3'- ccaCU-UC-GAAGA--AGCU-CGGGCCCCGa -5' |
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8208 | 3' | -56.7 | NC_001978.2 | + | 18899 | 0.7 | 0.350038 |
Target: 5'- --cGAAGCgcgcgcUCUUCGuGaCCGGGGCUu -3' miRNA: 3'- ccaCUUCGa-----AGAAGCuCgGGCCCCGA- -5' |
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8208 | 3' | -56.7 | NC_001978.2 | + | 24296 | 0.67 | 0.503231 |
Target: 5'- gGGcGGAGCaaugcgCUUCGuG-CCGGGGCUu -3' miRNA: 3'- -CCaCUUCGaa----GAAGCuCgGGCCCCGA- -5' |
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8208 | 3' | -56.7 | NC_001978.2 | + | 21321 | 0.66 | 0.56791 |
Target: 5'- aGUGAAGCccCUgggacaCGcGCCCGgGGGCUu -3' miRNA: 3'- cCACUUCGaaGAa-----GCuCGGGC-CCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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