Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8208 | 5' | -62.1 | NC_001978.2 | + | 30372 | 0.68 | 0.197935 |
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Target: 5'- --cGCgC-CGAAGCCGCCCGgcucGCGUg -3' miRNA: 3'- guuCGgGcGCUUCGGCGGGCa---CGCGa -5' |
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| 8208 | 5' | -62.1 | NC_001978.2 | + | 41152 | 0.69 | 0.172453 |
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Target: 5'- aGAGCCaUGCGAAGCggcgugcugcuauCGCCCGUGggaacaaCGCUg -3' miRNA: 3'- gUUCGG-GCGCUUCG-------------GCGGGCAC-------GCGA- -5' |
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| 8208 | 5' | -62.1 | NC_001978.2 | + | 11024 | 0.7 | 0.164337 |
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Target: 5'- uGGGC--GCGAAGUCGCCUGgGCGCUg -3' miRNA: 3'- gUUCGggCGCUUCGGCGGGCaCGCGA- -5' |
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| 8208 | 5' | -62.1 | NC_001978.2 | + | 15708 | 0.7 | 0.143569 |
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Target: 5'- uCGAGCCCcaGCGAAGCCGCCgGaacCGUc -3' miRNA: 3'- -GUUCGGG--CGCUUCGGCGGgCac-GCGa -5' |
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| 8208 | 5' | -62.1 | NC_001978.2 | + | 1680 | 0.7 | 0.143569 |
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Target: 5'- gCGAGCCCGaagcuuGCCGCC-GUGUGCg -3' miRNA: 3'- -GUUCGGGCgcuu--CGGCGGgCACGCGa -5' |
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| 8208 | 5' | -62.1 | NC_001978.2 | + | 12344 | 0.75 | 0.062127 |
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Target: 5'- gCGAGCCCGuUGAAGCCGUCCGcUGC-Cg -3' miRNA: 3'- -GUUCGGGC-GCUUCGGCGGGC-ACGcGa -5' |
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| 8208 | 5' | -62.1 | NC_001978.2 | + | 17626 | 0.75 | 0.062127 |
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Target: 5'- aCAAGCCCGCuGAGCCGUCCGga-GCc -3' miRNA: 3'- -GUUCGGGCGcUUCGGCGGGCacgCGa -5' |
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| 8208 | 5' | -62.1 | NC_001978.2 | + | 33040 | 1.07 | 0.000195 |
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Target: 5'- gCAAGCCCGCGAAGCCGCCCGUGCGCUa -3' miRNA: 3'- -GUUCGGGCGCUUCGGCGGGCACGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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