Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8209 | 3' | -55 | NC_001978.2 | + | 7345 | 0.66 | 0.67572 |
Target: 5'- gGCUCGAagGUcgccuucaagcaccuGGGUuGCGUGGcgACCGAa -3' miRNA: 3'- -CGAGCUaaCA---------------CCCGuCGCGCU--UGGCU- -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 12881 | 0.66 | 0.671259 |
Target: 5'- gGCUCGAUUGaguacGGGUgcgacGGCGUGAuugCGAa -3' miRNA: 3'- -CGAGCUAACa----CCCG-----UCGCGCUug-GCU- -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 36092 | 0.66 | 0.648877 |
Target: 5'- cGC-CGAcaccUGGGC-GCGCGAggaaGCCGAa -3' miRNA: 3'- -CGaGCUaac-ACCCGuCGCGCU----UGGCU- -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 22194 | 0.66 | 0.637656 |
Target: 5'- uGCUCGAc-GUaGGGCgucagaucAGCGCGcacacGGCCGAc -3' miRNA: 3'- -CGAGCUaaCA-CCCG--------UCGCGC-----UUGGCU- -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 33834 | 0.66 | 0.63092 |
Target: 5'- cGCUCGAcUGUcGGGCcgacggcguuacccgGGUGCcGACCGu -3' miRNA: 3'- -CGAGCUaACA-CCCG---------------UCGCGcUUGGCu -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 32688 | 0.67 | 0.61521 |
Target: 5'- --cCGGUUGUaGGCAGCGcCGGACaCGc -3' miRNA: 3'- cgaGCUAACAcCCGUCGC-GCUUG-GCu -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 7971 | 0.67 | 0.604004 |
Target: 5'- aGCUUGAUUGcGGGCGucacgccgucGCcCGggUCGAg -3' miRNA: 3'- -CGAGCUAACaCCCGU----------CGcGCuuGGCU- -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 40143 | 0.67 | 0.570577 |
Target: 5'- cGCuUCGAUcacGUGGGCgaAGcCGCcGACCGAc -3' miRNA: 3'- -CG-AGCUAa--CACCCG--UC-GCGcUUGGCU- -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 33404 | 0.68 | 0.51601 |
Target: 5'- -gUCGAUUGcc-GCAGCGuCGAGCUGAa -3' miRNA: 3'- cgAGCUAACaccCGUCGC-GCUUGGCU- -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 28828 | 0.69 | 0.484287 |
Target: 5'- --gCGcgUGUuGGCAGCGCucGCCGAc -3' miRNA: 3'- cgaGCuaACAcCCGUCGCGcuUGGCU- -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 16547 | 0.69 | 0.47392 |
Target: 5'- cGCgcgUGGUUG-GGGCGGCGUuucggcuGACCGAu -3' miRNA: 3'- -CGa--GCUAACaCCCGUCGCGc------UUGGCU- -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 4838 | 0.7 | 0.443514 |
Target: 5'- cGCUCuucaGUcGGCGGCGCGAacucACCGAu -3' miRNA: 3'- -CGAGcuaaCAcCCGUCGCGCU----UGGCU- -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 8602 | 0.7 | 0.414235 |
Target: 5'- gGCgCGAagcaucgGGGCacGGCGCGAACCGGc -3' miRNA: 3'- -CGaGCUaaca---CCCG--UCGCGCUUGGCU- -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 30010 | 0.73 | 0.27266 |
Target: 5'- -gUCGGcgGacUGGGCGGCGCGAAUCGu -3' miRNA: 3'- cgAGCUaaC--ACCCGUCGCGCUUGGCu -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 4606 | 0.86 | 0.035815 |
Target: 5'- uGCUCGAcgGUGGGCAGCGaCGGGCgGAc -3' miRNA: 3'- -CGAGCUaaCACCCGUCGC-GCUUGgCU- -5' |
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8209 | 3' | -55 | NC_001978.2 | + | 33302 | 1.11 | 0.000524 |
Target: 5'- aGCUCGAUUGUGGGCAGCGCGAACCGAa -3' miRNA: 3'- -CGAGCUAACACCCGUCGCGCUUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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